转CryIA(c)基因抗虫棉花棉籽的诱变活性研究  

Study on Mutagenicity of Cotton Seed Powder and Cotton Seed Oil of Transgenic CryIA(c)Cotton

作　　者：张宁[1] 陈明南[1] 马继霞[1] 李松岗[1] 许崇任[1] 

机构地区：[1]北京大学生命科学学院环境生物学及生态学系,北京100871

出　　处：《农业生物技术学报》2000年第3期217-221,共5页Journal of Agricultural Biotechnology

基　　金：国家863项目!编号:Z17-02-02

摘　　要：分别以环磷酰胺和氟化钠作为阳性诱变剂，以普通棉的棉籽粉及棉籽油作为对照物，同时设阴性对照组，以成年封闭群昆明种雄性小鼠和斑马鱼(Branchynanio rerio)为实验材料，将棉籽粉或棉籽油掺入饲料中饲喂小鼠或斑马鱼，检测小鼠骨髓嗜多染红细胞（Polychromatic erythrocytes, PCE）微核率（Micronucleus frequency,MN‰）和精子畸变率（Sperm malformation rate），以及斑马鱼外周血红细胞（Peripheral erythrocytes）微核率。结果表明：在本实验条件下，转CryIA(c)基因抗虫棉与普通棉的棉籽粉及棉籽粉油对小鼠体细胞和性细胞均未显示出诱变活性，且没有剂量效应。腹腔注射氟化钠可以诱导斑马鱼外周血红细胞微核率的显著升高；转CryIA(c)基因抗虫棉棉籽粉粉和普通棉棉籽粉粉一样，对斑马鱼外周血红细胞微核率无明显影响。鱼类饲料中添加常规量的转CryIA(c)基因抗虫棉棉籽粉不会诱发微核产生。The mutagenicity of seed powder and seed oil of transgenic C ryIA(c) cotton to mammals and fish was evaluated in this study. The ra w cotton seed powder or seed oil was mixed into mice and fish's diet. Cyclophosphamide and NaF were used as positive controls respectively. Regular cotton seed powder and seed oil were used as negative controls . The micronucleus frequency in mice bone marrow polychromatic erythro cytes and in eripheral erythrocytes of Zebrafish(Branchynanio rerio)we re assayed in this study. The sperm malformation rate in mice was also investigated. The results showed that, the seed powder and seed oil o f transgenic CryIA(c) cotton lines and regular cotton showed no cytomu tagenicity or genomutagenicity on mice. No dose effect was observed. N aF can induce significant increase of micronucleus frequency in eriphe ral erythrocytes of Zebrafish. Neither transgenic CryIA(c) cotton see d powder nor regular cotton seed powder has obvious effect on micronuc leus frequency in eripheral erythrocytes of Zebrafish. The use of com mon proportion of transgenic CryIA cotton seed powder in fish's feed d oes not induce the increase of micronucleus frequency obviously.

关 键 词：Bt毒蛋白抗虫棉 小鼠 斑马鱼 微核率 精子畸变率 

分 类 号：S565.034[农业科学—作物学]