通过AFLP-DNA指纹的计算机分析进行水稻种子鉴定  

Rice Seed Identification by Computerized AFLP-DNA Fingerprinting Analysis

作　　者：陈一华[1] 贾建航[1] 李传友[1] 金德敏[1] 翁曼丽[1] 王斌[1] 

机构地区：[1]中国科学院遗传研究所,北京100101

出　　处：《农业生物技术学报》2000年第3期222-224,共3页Journal of Agricultural Biotechnology

基　　金：863项目

摘　　要：本研究用１２对引物组合对杂交水稻生产中广泛应用的优良杂交组合及其亲本（共１５个材料）进行了ＡＦＬＰ分析。通过优选，筛选出 ３对引物，即 Ｅ－ＡＣＴ／Ｍ·ＣＡＣ， Ｅ－ＡＡＣ／Ｍ－ＣＡＡ及 Ｅ－ＡＡＧ／Ｍ－ＣＡＡ，它们是本研究中用来区分所研究的 １５个材料所需的最少引物组合。然后根据所选用的１６个多态性ＤＮＡ条带的有无，把每个材料的ＤＮＡ指纹转换成由１和０组成的指纹数据。在此基础上，开发了一套应用软件。在实际应用中，将样品的扩增结果转换成指纹数据，由计算机辅助进行种子鉴定。AFLP analysis of 15 rice varieties that were widely used in rice production in China was carried out 3 primer-pairs, i. e. E-ACT/M-CAC, E-AAC/M-CAA 及 E-AAG/M-CAA were screened out. They were the minimum number of primer pairs to be used to distinguish the 15 analyzed rice varieties. DNA fingerprints of the 15 varieties were developed based on 16 selected band amplified by the above three primer-pairs. The DNA fingerprint of each variety was converted into a computerized fingerprint constituted of 1 and 0 based on the presence or absence of the DNA band. In the practical seed identification, the AFLP-DNA fingerprint of a sample was converted into the computerized fingerprint, which can be processed by the software SIR designed by us, so the seed identitication can be completed by the aid of a computer.

关 键 词：种子鉴定 AFLP-DNA指纹分析 计算机处理 水稻 

分 类 号：S511.031[农业科学—作物学] S126