溶血反应对高血压患者肾素-血管紧张素-醛固酮系统检测的影响  

The effect of haemolytic reaction on the detection of renin-angiotensin-aldosterone system in hy- pertension patients

作　　者：邵姣梅[1] 刘钰涛[1] 唐家荣[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院,武汉430030

出　　处：《临床内科杂志》2013年第1期18-20,共3页Journal of Clinical Internal Medicine

摘　　要：目的探讨溶血反应对高血压患者血浆中肾素活性（PRA）、血管紧张素Ⅰ（AngⅠ）、血管紧张素Ⅱ（AngⅡ）、醛固酮（ALD）及醛固酮／肾素活性比值（ARR）检测结果的影响。方法将60例高血压患者血标本每例分成2份，每例标本各取1份施行模拟溶血，将溶血及非溶血样本离心后，分别取血浆，应用放射免疫法检测血浆PRA、AngⅠ、AngⅡ、ALD及ARR水平，并对溶血组及非溶血组结果进行统计分析。结果溶血样本PRA、AngⅠ、AngⅡ、ALD水平均明显低于非溶血样本（P〈0．01），ARR假阳性率升高（P〈0．05）。结论溶血对血浆PRA、AngⅠ、AngⅡ、ALD及ARR检测结果有显著影响，严重干扰临床诊断，在实际工作中应予以重视。Objective To explore whether the hemolytic reaction will influence the test results of plasma renin activity（PRA） ,angiotensin Ⅰ （ Ang Ⅰ ） ,angiotensin Ⅱ （ Ang Ⅱ ） ,aldosterone（ALD） and the aldosterone/plasma renin activity ratio（ARR） in hypertension patients. Methods Each blood sample of the 60 cases was divided into 2 tubes, one of which was performed simulate hemolysis. All samples were centrifuged in 2500 rpm for 8 min to divide plasma. Plasma PRA, Ang Ⅰ , Ang Ⅱ , ALD and ARR levels were detected by using radioimmunoassay and analysed. Results Compared with non-hemolysis plasma, the content of PRA, AⅠ, Ang Ⅱ, ALD in hemolysis plasma were obviously lower（ P 〈 0.01 ） , and the false- positive rate of ARR rose in hemolysis samples （ P 〈 0.05 ）. Conclusions The five test results （ PRA, Ang Ⅰ , Ang Ⅱ , ALD and ARR） were seriously interfered with hemolysis. We should pay more attention to avoid hemolytic reaction in clinical practice.

关 键 词：溶血 肾素活性 醛固酮 血浆醛固酮 肾素活性比值 

分 类 号：R541.61[医药卫生—心血管疾病]