体外诱导泛耐药铜绿假单胞菌与临床分离株产金属β-内酰胺酶差异分析  

Establishment of pandrug-resistant Pseudomonas aeruginosa model in vitro and detection with metallo-β-lactamasee

作　　者：蔡兴俊[1] 吴华[2] 黄奕江[1] 莫濡冰[1] 

机构地区：[1]海南省人民医院呼吸内科,海口570311 [2]海南省人民医院检验科,海口570311

出　　处：《临床内科杂志》2013年第1期57-58,共2页Journal of Clinical Internal Medicine

基　　金：海南省自然科学基金项目资助（808203）

摘　　要：目的通过建立泛耐药铜绿假单胞菌体外模型，探讨诱导株与临床分离株产金属β-内酰胺酶的差异。方法应用多种抗菌素次抑菌浓度诱导法建立体外耐药模型，应用纸片协同法检测诱导株与临床分离株产金属β-内酰胺酶。结果12株临床分离敏感株经多种抗菌素次抑菌浓度逐步诱导，全部转为泛耐药株；应用双纸片协同法检测临床分离株产金属β-内酰胺酶比率为52．6％（20／38），体外诱导株为16．7％（2／12），两组产酶率比较差异有统计学意义（P〈0．05）。结论应用多种抗菌素次抑菌浓度逐步诱导法可成功建立体外耐药铜绿假单胞菌模型；次抑菌浓度体外诱导株产金属β-内酰胺酶比率低，并非主要耐药机制。Objective To explore the differences of producing metallo-β-1actamase between in- duced strains and clinical isolated strains by establishing pandrug-resistant pseudomonas aeruginosa model in vitro. Methods To establish drug-resistant model in vitro by inducing method through using multiple antibiotics with sub-inhibitory concentrations ; disc synergy test was used to detect the production of metal- lo-β-1actamases between induced strains and clinical isolated strains. Results 12 clinical isolated-sensi- tive strains all converted to PDR strains by inducing method of multiple antibiotics with sub-inhibitory con- centrations. The ratios of producing metallo-β-lactamase in clinical isolated strains and in vitro induced strains detected by double-disc synergy test were 20/38 （52.6%） and 2/12 （ 16.7% ） ,respectively,the difference of the enzyme production rate was significant between these two groups （ χ2 = 4. 788, P 〈 0.05）. Conclusion Inducing method of multiple antibiotics with sub-inhibitory concentrations can be successfully used to establish drug-resistant model in vitro;The ratio of producing metallo-β-1actamase in induced strains in vitro was low, and it is not the major mechanism of drug-resistance.

关 键 词：铜绿假单胞菌 泛耐药 次抑菌浓度 金属Β-内酰胺酶 

分 类 号：R378.991[医药卫生—病原生物学]