多胺对白桦悬浮细胞生长和三萜积累的影响  

Effect of polyamine on growth of Betula platyphylla suspension cells and triterpenoid accumulation

作　　者：李晓灿[1] 詹亚光[1] 王晓东[1] 刘英甜[1] 李丹[1] 荣令珍[1] 范桂枝[1] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《中草药》2013年第4期463-467,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(31100445;31070531);中央高校基本科研业务专项资金项目(DL12CA05);黑龙江省博士后科研启动金(415288)

摘　　要：目的分析外源多胺对白桦悬浮细胞生长和三萜积累的影响。方法在白桦悬浮细胞的生长末期添加0.1 mmol/L和1.0 mmol/L的腐胺(Put)、精胺(Spm)和亚精胺(Spd),采用比色法和RT-PCR方法分析白桦三萜量及其合成关键酶羽扇豆醇合酶(LUS)基因的表达。结果除1.0 mmol/L Spm处理使白桦悬浮细胞的活力和干质量积累降低外,其他处理均提高了白桦悬浮细胞的活力、干质量积累和三萜产量,且随着处理时间的延长,干质量积累和三萜产量呈增加趋势。其中1.0 mmol/L Put在处理的第2天对细胞干质量和三萜产量的促进效应最大,分别比对照增加了38.89%和116.35%。LUS基因的RT-PCR检测结果进一步证实了多胺对白桦三萜积累的促进作用。结论 Put可有效促进白桦悬浮细胞生长和三萜积累。Objective To analyze the effect of exogenous polyamine on the growth of Betula platyphylla suspension cells and triterpenoid accumulation. Methods At the end of the growth stage, 0.1 mmol/L and 1.0 mmol/L putrescine （Put）, spermine （Spm）, and spermidine （Spd） were added to the suspension cells of B. platyphylla, the triterpenoid content and gene expression of lupeol synthase （LUS） related to the synthesis of triterpenoid were analyzed by chemical colorimetry and RT-PCR, respectively. Results Except for the treatment with 1.0 mmol/L Spm, the cell viability, dry weight, and triterpenoid production in B. platyphylla suspension cells induced by Put and Spd were increased, and the dry weight and triterpenoid production ofB. platyphylla cells were increasing with the extension of polyamine treatment time. Among them, dry weight and triterpenoid production were the highest after 2 d Put induction, which were 38.89% and 116.35% higher than those in the control, respectively. Triterpenoid production in B. platyphylla cells induced by polyamine was further confirmed by RT-PCR ofLUS gene. Conclusion Put could effectively enhance the growth of B. platyphylla suspension cells and triterpenoid accumulation.

关 键 词：白桦 悬浮细胞 三萜 多胺 羽扇豆醇合酶 

分 类 号：R282.21[医药卫生—中药学]