昆明小鼠胃肠道钙离子跨膜吸收途径相关基因表达模式分析  

Expression Patterns Analysis of Calcium Ion Trans-Membranous Absorption Related Genes in Gastrointestinal Tract of Kunming Mice

作　　者：杨艺[1] 莘海亮[1] 夏先林[1] 吴文旋[1] 李胜利[2] 

机构地区：[1]贵州大学动物科学学院,贵阳550025 [2]中国农业大学动物科技学院,动物营养学国家重点实验室,北京100193

出　　处：《动物营养学报》2013年第2期441-446,共6页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金(31160468;30901039);动物营养学国家重点实验室开放课题(2004DA125184F1115)

摘　　要：本研究旨在分析钙离子(Ca2+)跨膜吸收途径相关基因在昆明小鼠胃肠道中的表达模式。选取12只8周龄、平均体重(30.71±2.93)g的雌性昆明小鼠,取胃、十二指肠、空肠、回肠、盲肠和结肠组织样品,利用实时定量PCR法检测维生素D依赖性钙结合蛋白(CaBP-D9k)、瞬时性受体电位通道香草酸受体6(TRPV6)和维生素D受体(VDR)mRNA表达量。结果表明:1)CaBP-D9k、TRPV6和VDR mRNA在胃内属低水平表达,而在盲肠内表达量较高;2)随着小肠的延伸,CaBP-D9k、VDR mRNA表达量逐渐降低,而TRPV6 mRNA则在回肠内高水平表达;3)CaBP-D9k(P<0.05)、VDR(P<0.05)、TRPV6 mRNA的表达量(P>0.05)随着大肠肠段的延伸而不同程度地下降。结果提示,CaBP-D9k、TRPV6和VDR mRNA表达量与胃肠道Ca2+跨膜吸收能力存在关联性。This study was conducted to analyze the expression patterns of calcium ion trans-membranous ab- sorption related genes in gastrointestinal tract of Kunming mice. A total of 12 female Kunming mice aged 8 weeks with an average body weight of （30.71 ±2.93 ） g were used as experimental animals. Tissues of stom- ach, duodenum, jejunum, ileum, cecum and colon were collected for the analysis of vitamin D-dependent 9 ku calcium-binding protein （ CaBP-D9k）, transient receptor potential vanilloid receptor 6 （ TRPV6）, and vita- min D receptor （VDR） mRNA expression levels by RT-qPCR. The results showed as follows ： 1 ） CaBP- Dgk, TRPV6 and VDR mRNA expressed in stomach at a low level, but in cecum at a high level; 2 ） with the extension of small intestine, expression levels of CaBP-D9k and VDR mRNA were gradually reduced, while TRPV6 mRNA expressed in ileum at a high level; 3 ） with the extension of large intestine, expression levels of CaBP-D9k （P 〈0.05）, VDR （P 〈0.05） and TRPV6 mRNA （P 〉0.05） were also gradually reduced at dif- ferent levels. These results indicate that expression levels of CaBP-D9k, VDR and TRPV6 mRNA are associat- ed with the capability of Ca2＋ trans-membranous absorption in gastrointestinal tract.

关 键 词：胃肠道 钙离子跨膜吸收 维生素D依赖性钙结合蛋白 瞬时性受体电位通道香草酸受体6 维生素D受体 昆明小鼠 

分 类 号：S852.2[农业科学—基础兽医学]