冠心病心肌细胞凋亡的研究  

Study on cardiomyocyte apoptosis in coronary heart disease by TUNEL/TEM method

作　　者：罗斌[1] 马中富 罗质人[2] 汪冠三[2] 王穗保[2] 徐小虎[3] 宋一璇[1] 高修仁 许心舒[2] 林俊莲[2] 

机构地区：[1]中山医科大学法医学系,广州510089 [2]广州市公安局刑事科学研究所,广州510030 [3]广东汕头大学医学院,汕头515031

出　　处：《中国法医学杂志》2000年第B06期18-20,共3页Chinese Journal of Forensic Medicine

基　　金：国家自然科学基金(39700167;39170396);广东省医学科研基金;广东省五个一科教兴医工程专项基金

摘　　要：探索细胞凋亡在心血管系统的意义,应用TUNEL法、透射电镜技术观察20例冠心病猝死者梗死区心肌细胞凋亡现象。冠心病组梗死区心肌细胞凋亡千分率(557.94±144.10％)明显高于正常对照组(34.30±20.68％),P<0.01,并有趋势表明,冠状动脉粥样硬化病变支数越多,梗死区心肌细胞凋亡越明显。电镜下见有的心肌细胞出现胞核体积缩小、染色质边聚、核膜完整的早期凋亡特征;有的则出现染色质更浓密、电子密度明显增加、结构模糊不清、核膜仍完整的中晚期凋亡细胞特征。细胞凋亡在冠心病心肌梗死是一种相当重要的现象。In order to explore the significance of cardiomyocyte apoptosis in coronary heart disease (CHD), the TUNEL and transmission electron microscopic (TEM) techniques were performed. The results showed that the permillage of cardiomyocyte apoptosis in infarct area in patients of sudden death from CHD was significantly higher than that in control group (557.94± 144.10‰ VS 34.30±20.68‰, p<0.01). The TEM showed the characteristics of early apoptosis episodes, including nuclear shrinkage, marginalt distribution of chromatin, and intact nuclear membrane of cardiomyocytes. Some cardiomyocytes showed condensed chromatin, increased electron density, hazy structure, and intact nuclear membrane which are signs of middle and late stages of apoptosis. Cardiomyocyte apoptosis is the characteristics in infarct in patients with CHD.

关 键 词：冠心病 细胞凋亡 TUNEL检测法 

分 类 号：D919[医药卫生—法医学]