正常成人和成体大鼠气管软骨组织动力学观察  

Study on histodynamics of tracheal cartilage

作　　者：史学义[1] 丁一[1] 宗安民[1] 冯密罗[2] 张林庆[1] 

机构地区：[1]河南医科大学组织学与胚胎学教研室,郑州450052 [2]河南医科大学物理学教研室,郑州450052

出　　处：《河南医科大学学报》2000年第4期315-317,F003,共4页Journal of Henan Medical University

基　　金：河南省教委自然科学基础研究项目!19993 10 0 0 8

摘　　要：目的 :观察正常成人和成体大鼠气管软骨的组织动力学。方法 :应用细胞生物学方法、衰老相关 β 半乳糖苷酶组织化学染色和增殖细胞核抗原免疫组织化学方法 ,研究正常成人和正常成体大鼠气管软骨组织动力学。结果 :软骨细胞呈现多种方式的无丝分裂像 ;气管软骨膜与软骨周围带多见细胞增生 ;气管软骨中央带多见衰老的软骨细胞 ,并见明显核固缩、核碎裂等细胞凋亡形态学特征。结论 :从软骨膜最外层致密结缔组织到成熟软骨组织呈现一个连续的组织演化动力学过程 ;Aim: To study the histodynamics of the normal tracheal cartileage. Methods: cell biological assay, histochemical staining of senescence associated β galactosidase and immunohistochemical staining of proliferating cell nuclear antigen were used. Results: chondrocytes exhibited various types of amitotic figures. The proliferating cells were mainly distributed in the perichondrium and the peripheral zone of tracheal cartilage. The senescent chondrocytes were distributed in the central zone where apoptotic morphological characteristics obviously appeared, such as pyknosis, karyorrhexis. Conclusion: The evolution from dense connective tissue of perichondrial outmost layer to mature cartilaginous tissue showed a continuous evolving histokinetic process. The tracheal perichondrium was the originating site of adaptive cartilage regeneration.

关 键 词：成人 大鼠 气管 软骨 细胞凋亡 组织动力学 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R332.2[医药卫生—基础医学]