高效液相色谱荧光检测法测定辣椒制品中罗丹明B的含量  

Quantitative determination of Rhodamine B in chilli products by HPLC-FLD

作　　者：杨仁惠[1] 胡立立[1] 许乾丽[2] 史蕙[2] 吴俣[3] 王烈[3] 杨蝉[3] 

机构地区：[1]贵阳医学院药学院,贵州贵阳550004 [2]贵州省食品药品检验所,贵州贵阳550004 [3]贵阳中医学院,贵州贵阳550002

出　　处：《现代医药卫生》2013年第4期481-482,485,共3页Journal of Modern Medicine & Health

基　　金：贵州省科技厅资助项目[黔科合SY字(2011)3090号]

摘　　要：目的建立辣椒制品中非法添加化工染料罗丹明B含量的测定方法。方法采用高效液相色谱荧光检测(HPLC-FLD)器,色谱柱:Waters XBridgeTM苯基柱(5μm,4.6 mm×150 mm);流动相:甲醇-含0.2%甲酸和0.03%三乙胺的水溶液梯度洗脱;检测波长:激发波长550 nm(Ex),发射波长580 nm(Em);流速:1.0 mL/min。结果罗丹明B进样量在0.050 74～1.268 50μg范围内线性关系良好(r=0.999 7),平均回收率为97.2%,相对标准偏差(RSD)为1.7%。结论该法简便、准确、重现性好,可用于辣椒制品中非法添加物罗丹明B含量的测定。Objective To establish a method for the quantitative determination of illegally added chemical dye Rhodamine B in the chilli products. Methods The HPLC-FLD method was performed on the Waters XBridgeTM phenyl column （5 μm,4.6 mm×l50 mm） with the mobile phase of methanol--aqueous solution containing 0.2% formic acid and 0.03% triethy- lamine for gradient elution.The FLD excitation wavelength and emission wavelength were set at 550 nm（λEx） and 580 nm（λEm） ; and the flow rate was 1.0 mL/min. Results The linear range of rhodamine B was 0.050 74-1.268 5 p,g （r=0.999 7 ）.The mean recovery rate was 97.2% and RSD was 1.7%. Conclusion This method is simple and accurate with good reproducibility, and can be used for the determination of illegally added Rhodamine B content in the chilli products.

关 键 词：色谱法 高压液相 光谱法 荧光 辣椒属 罗丹明B 食品检查 

分 类 号：R969.1[医药卫生—药理学]