FSH对绵羊卵母细胞体外发育能力的影响  

Effect of FSH on the in vitro developmental compentence of sheep oocytes

作　　者：赵驻军[1,2] 李瑞岐[1,3] 莫显红[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071000 [2]河北省牛羊胚胎工程技术研究中心,河北保定071000 [3]中山大学孙逸仙纪念医院妇产科生殖医学中心,广州510120

出　　处：《黑龙江畜牧兽医》2013年第3期38-40,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金："十二五"国家科技支撑计划项目(2011BAD19B02);农业科技成果转化资金项目(2011GB2A210004);河北省自然科学基金项目(C2010000786)

摘　　要：为了探讨绵羊卵母细胞体外成熟过程中促卵泡素(FSH)的适宜添加量,试验将FSH分为0.01,0.02,0.03 IU/mL 3个浓度梯度,用于绵羊卵母细胞的体外成熟与体外受精,统计第一极体排出率、卵裂率和囊胚率。结果表明:不同浓度FSH对第一极体排出率影响差异不显著(P>0.05),体外受精后以0.02 IU/mL组效果最好,卵裂率显著高于0.03 IU/mL组(P<0.05),但与0.01 IU/mL组相比差异不显著(P>0.05);0.02 IU/mL组囊胚率与其他2组相比显著升高(P<0.05)。To investigate the appropriate doe of FSH in sheep oocyte maturation in vitro, the concentration of FSH were divided into 3 concentration gradients （ including 0.01 IU/mL, 0.02 IU/mL and 0.03 IU/mL） for in vitro maturation and in vitro fertilization of sheep oocytes, and then the rates of polar body I （ Pb I ） expulsion, cleavage and blastocyst development were analyzed respectively. The results showed that there were no significant effects of different concentrations of FSH on rate of Pb I expulsion among the three groups （ P 〉 0.05 ）. The 0.02 IU/mL group had the best effect on the cleavage rate after in vitro fertilization, this rate was significantly higher than that in the 0.03 IU/mL group（ P 〈 0.05 ）, but there were no significant differences compared to the 0.01 IU/mL group（ P 〉 0.05 ）. The rate of blastocyst development was significantly higher in the 0.02 IU/mL group compared to the other two groups（ P 〈 0.05 ）.

关 键 词：促卵泡素(FSH) 卵母细胞 体外成熟 体外受精 绵羊 

分 类 号：S814.1[农业科学—畜牧学]