猪源戊型肝炎病毒ORF3基因的原核表达及抗原性分析  

Prokaryotic expression and antigenic analysis of the ORF3 gene of swine hepatitis E virus

作　　者：梁焕斌[1] 梁楚敏[1] 古洪浪[1] 张亮权[1] 王衡[1] 张桂红[1] 

机构地区：[1]华南农业大学兽医学院农业部动物疫病防控重点实验室及广东省动物源性人畜共患病预防与控制重点实验室,广州510642

出　　处：《黑龙江畜牧兽医》2013年第3期86-88,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：现代农业产业技术体系建设专项资金项目(CARS-36);高等学校博士学科点专项科研基金项目(20104404110009;20114404120010)

摘　　要：为了研究猪源戊型肝炎病毒(swHEV)ORF3部分基因的抗原性,试验通过设计引物,扩增swHEV ORF3部分基因片段,使用pMD18-T载体克隆,双酶切后插入pET-32a(+)表达载体,质粒经鉴定为阳性后,转化大肠杆菌BL21,并进行IPTG诱导,SDS-PAGE凝胶电泳和Western-blot检测。结果表明:获得目的蛋白约35 ku,该蛋白具有良好的抗原性,以可溶性形式存在。To study the antigenicity of part of ORF3 gene from swine Hepatitis E virus （ swHEV）, a partial gene fragment of the ORF3 from swHEV was amplified by using a pair of specific primers designed. The fragment was cloned into pMD18 - T vector, and was inserted into pET -32a（ ＋ ） vector after double digestion, and then the recombinant plasmid was transformed into E. coil BL21. After IPTG induction, the protein was analyzed by SDS - PAGE and Western - blot. The result showed that the target protein was obtained and its molecular weight was estimated to be 35 ku. It was found that the protein had good antigenieity and existed mainly in soluble form,

关 键 词：猪源戊型肝炎病毒 ORF3基因 原核表达 

分 类 号：S852.65[农业科学—基础兽医学]