高温处理对转基因作物种子Bt蛋白含量的影响  

Impact of High Temperature Treatment on the Content of Bt Protein in Genetically Modified Crop Seeds

作　　者：张坤[1] 谭桂玉[1] 王保民[1] 南铁贵[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193

出　　处：《生物技术通报》2013年第2期76-79,共4页Biotechnology Bulletin

基　　金：转基因生物新品种培育重大专项(2009ZX08012-017B);国家自然科学基金项目(31271649)

摘　　要：转基因作物食品安全问题日益受到社会关注,作物种子作为食物的重要来源之一,转基因作物种子的安全性研究更有待加强。分别利用转Bt基因棉花和玉米品种及非转基因品种种子进行高温处理(55-130℃),并用双抗夹心酶免疫检测方法(ELISA)研究其中的外源Bt蛋白的动态变化过程。结果表明,不同作物种子中的Bt蛋白对高温的耐受程度不同,棉花种子中Bt蛋白较玉米种子耐高温能力强;高温处理条件下,种子中的Bt蛋白在10 min内含量显著下降,其后降解缓慢;130℃以上的高温处理条件下,玉米和棉花种子中的外源Bt蛋白均能显著降解。Food safety problem of genetically modified crops have aroused public concern day by day. As crop seed is one of the most important source of food, the research on safety of genetically modified crop seeds need to be strengthened. The present work applied cotton and corn seeds, Bt modified varieties and non-genetically modified varieties, to treat with high temperature （ 55-130℃ ）, and then detected the dyna- mic digestion process of Bt protein with sandwich enzyme link immunoassay （ ELISA ） . The result of the assay showed that the Bt protein in different crops have different degree of tolerance to high temperature. The capacity of enduring heat stress of Bt protein in cotton seed is better than that in corn seed ; the toxin protein significantly decreased within 10 min in the high temperature treatment, followed by slow degradation ; all Bt protein in transgenic corn and cotton seeds can obviously inactivation treated with 130℃ or higher temperature.

关 键 词：高温 BT蛋白 种子 双抗夹心酶联免疫吸附检测方法 

分 类 号：S188[农业科学—农业基础科学]