甘蔗脱落酸胁迫成熟诱导蛋白基因(SoASR)的克隆和表达分析  

Molecular Cloning of Sugarcane ASR Gene ( SoASR ) and Its Expression Analysis

作　　者：黄杏[1,2] 杨丽涛[1,2] 张保青[1] 宋修鹏[1] 李杨瑞[1,2] 王盛[1] 

机构地区：[1]广西大学农学院亚热带农业生物资源保护与利用国家重点实验室,南宁530005 [2]中国农业科学院甘蔗研究中心农业部广西甘蔗生物技术与遗传改良重点实验室广西作物遗传改良生物技术重点实验室广西甘蔗遗传改良重点实验室,南宁530007

出　　处：《生物技术通报》2013年第2期93-99,共7页Biotechnology Bulletin

基　　金：科技部国际合作项目(2009DFA30820);广西自然科学基金创新团队项目(2011GXNSFF018002);广西科学研究与技术开发计划项目(桂科产1123008-1);广西农科院团队项目(桂农科2011YT01)

摘　　要：采用同源克隆和RT-PCR技术克隆了甘蔗SoASR基因全长cDNA,GenBank登录号为JX470187,长度为753 bp,包括一个429 bp的开放阅读框,编码142个氨基酸的蛋白。同源性分析表明,甘蔗SoASR与大蕉、玉米和高粱聚为一小组,同源性分别为79%、93%和88%。将SoASR在大肠杆菌中表达,获得一个约32.0 kD的外源蛋白。实时荧光定量PCR分析结果表明,低温胁迫下该基因在抗寒性强的甘蔗品种桂糖28号(GT28)中表达量先升后降,在抗寒性弱的甘蔗品种园林6号(YL6)中则呈下降趋势;该基因的表达在两个甘蔗品种中均受ABA的诱导。这说明SoASR基因在甘蔗抗寒机制中发挥一定作用。In this study, the full length of SoASR cDNA obtained by homologous cloning and RT-PCR. It consists of 753 bp with an open reading frame of 429 bp, encoding a polypeptide of 142 amino acids, and its GenBank accession number is JX470187. Homology analysis showed that SoASR were clustered into a group with barley, maize and sorghum with homology of 79%, 93% and 88%, respectively. A 32.0 kD heterologous protein was obtained when SoASR gene was expressed in E. coli. The results of quantitative real-time PCR analysis showed that, the mRNA of ASR was first up-regulated and then down-regulated in the sugarcane variety with strong cold resistance, GT28, and down-regulated in the sugarcane variety with weak cold resistance, YL6, under low temperature stress. The expressions of SoASR gene were induced in two varieties. These results suggested that SoASR might be involved in response to cold stress in sugarcane.

关 键 词：甘蔗 脱落酸胁迫成熟诱导蛋白 克隆 表达 

分 类 号：S566.1[农业科学—作物学]