山苍子内生真菌多样性研究  

On Diversity of Endophytic Fungi Isolated from Litsea Cubeba

作　　者：游玲[1] 林娜[2] 郭华[2] 廖雪丽[2] 魏琴[1] 

机构地区：[1]宜宾学院 发酵资源与应用四川省高校重点实验室,四川宜宾644000 [2]宜宾学院生命科学与食品工程学院,四川宜宾644000

出　　处：《西南师范大学学报（自然科学版）》2013年第1期121-126,共6页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：四川省科技厅青年基金项目(2011JTD0035);四川省教育厅青年基金项目“山苍子内生菌分离及应用研究”(2009JY0149);发酵资源与应用四川省高校重点实验室基金项目(No.2010KFJ002)

摘　　要：采用添加植物组织浸提液的孟加拉红培养基,从山苍子根、茎、叶、果实中分离到25株内生真菌,其中根内生真菌5株,茎内生真菌3株,叶内生真菌12株,果实内生真菌5株,经形态及ITS序列鉴定,其至少分属于As-pergillus、Cladosporium、Hypocrea、Penicillium4个属的11个种,且Hypocrea属的3株真菌仅在叶、果实中分离到,Cladosporium属的1株真菌仅在叶中分离到,表明山苍子植株对其内生真菌具有明显的选择作用,其内生真菌的分布体现出明显的种属特异性及组织特异性.The rose bengal medium added sterilized aqueous extract of Litsea cubeba plant tissue has been used as isolation medium, 25 endophytic fungal strains have been isolated from surface--sterilized leaves, stems, roots and fruits of Litsea cubeba in Yibin, Sichuan province, China, and 5 strains from roots, 3 strains from stems, 12 strains from leaves, 5 strains from fruits. These strains have been classified into Aspergillus, Cladosporiurn, Hypocrea, Penicilliurn containing at least 11 species, and 3 strains belong to Hypocrea were only isolated from leaves and fruits, 1 strains belong to Cladosporium have been only isola- ted from leaves. The results indicate that Litsea cubeba had considerable select on its endophytic fungi col lection; both species specificity and tissue specificity have been observed in distribution of endophytic fungi of Litsea cubeba

关 键 词：山苍子 内生真菌 多样性 

分 类 号：Q935[生物学—微生物学]