植物皂苷生物合成中UGT功能研究进展  

Research Progresses on UGTs Involved in Biosynthesis of Plant Saponins

作　　者：陶韵文[1,2] 徐洁森 孙晶[2,3] 魏建和 隋春 刘娟[1] 

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007 [2]中国医学科学院&中国协和医学院药用植物研究所,北京100193 [3]东北林业大学生命科学院,黑龙江哈尔滨150040

出　　处：《药物生物技术》2013年第1期85-90,共6页Pharmaceutical Biotechnology

基　　金：国家自然科学基金面上项(No.81072994);北京市自然科学基金面上项目(No.5102033);中医药行业科研专项(No.201107011)

摘　　要：植物皂苷(Saponins)是广泛存在于绝大多数植物体中不可或缺的一类次生代谢产物。其中多种植物皂苷具有重要的药理活性。尽管皂苷的生物合成途径还未彻底解析,但近年来,皂苷生物合成途径研究,尤其是合成途径下游多基因家族编码酶催化的反应,取得了很大进展。目前,已在个别植物中克隆到了催化个别单体皂苷生物合成的UGT基因,其功能研究取得了一定进展。本文就皂苷合成途径下游催化皂苷元糖基化的糖基转移酶基因克隆和功能研究做一综述,为加快皂苷生物合成途径的解析提供借鉴。Saponins is an indispensable kind of secondary metabolites, which widely exists in plant kingdom. Many kinds of plantsaponins have important pharmacological activity. Although the complete biosynthetic pathway of saponin has not been elucidated in any single species,the researches on the saponins biosynthesis have made great progress in recent years, especially on the enzyme involved in the downstream of pathway and coded by multigene families. At present, the UGT genes of some individual plants have been cloned to catalytic the biosynthesis of individual monomer saponin. The function researches of the UGT genes have made certain progresses. In this paper,we reviewed the progresses on the gene cloning and functioning researches for the UDP-glycosyltransferases, which participate in the downstream of saponin biosynthetic pathway to catalyze the glycosylation of sapogenins. The aim of present paper is to provide some help for promoting the elucidation of the saponin biosynthetic pathway.

关 键 词：植物皂苷 生物合成 UDP-糖基转移酶(UGT) 基因克隆 晶体结构 研究进展 

分 类 号：R284.3[医药卫生—中药学]