干燥综合征泪腺组织中细胞凋亡及相关基因表达的研究  

Apoptosis and related gene expression in lacrimal gland of cases with Sjgren′s syndrome

作　　者：吴军[1] 费佩芬 龚伊红[1] 赵家良[1] 董继红[1] 唐福林[1] 

机构地区：[1]中国医学科学院

出　　处：《中华眼科杂志》2000年第4期255-258,I014,共5页Chinese Journal of Ophthalmology

摘　　要：目的　探讨干燥综合征 (Sj gren′ssyndrome,SS)患者泪腺组织上皮细胞凋亡及其相关基因表达与SS组织损伤的关系。方法　采用原位末端标记及免疫组织化学的方法 ,对 12例SS患者及7例对照泪腺组织上皮细胞中的凋亡细胞及Fas、FasL、bcl 2和Bax等相关基因蛋白的表达进行检测。结果　 12例SS患者泪腺组织中上皮细胞凋亡数为 (8 83± 3 2 7)个 /高倍视野 ;对照为 (0 88± 0 5 2 )个 /高倍视野 (P =0 0 0 0 )。SS患者泪腺导管及腺泡上皮细胞中 ,Fas、FasL及Bax阳性表达的细胞数均明显高于对照组 (P =0 0 0 0 ) ,与凋亡细胞数呈正相关 (R =0 83,0 88,0 8;P =0 0 0 0 ) ;bcl 2阳性表达的细胞数明显低于对照组 ,与凋亡细胞数呈负相关 (R =- 0 6 9,P =0 0 0 1)。结论　SS患者泪腺组织中上皮细胞凋亡可能是导致腺体破坏进而功能丧失的原因之一 ;Fas、FasL及Bax增加以及bclObjective To assess the roles of apoptosis and expression of the related genes in lacrimal glandular destruction of patients with Sjgren′s syndrome(SS) Methods Small pieces of palpebral lobes of lacrimal glands were obtained from 12 patients (10 with primary Sjgren′s syndrome, 2 with secondary Sjgren′s syndrome) and 7 normal controls They had been investigated by the in situ end labeling and immunohistochemical staining to detect the apoptotic cells and the expression of Fas, FasL, bcl 2 and Bax Results The number of epithelial apoptotic cells in lacrimal glands of patients with SS was significantly higher than that in normal glands, and SS epithelial cells showed increased expression of Fas, FasL and Bax There was significant positive correlation between the number of the apoptotic cells and that of the cells expressing Fas, FasL and Bax, respectively, and there was significant negative correlation between the number of the apoptotic cells and that of the cells expressing bcl 2 Conclusions The apoptosis of the epithelial cells in the lacrimal gland may be one of the mechanisms leading to the glandular destruction found in SS In SS lacrimal glands, the expression of Fas, FasL and Bax may promote apoptosis, and the expression of bcl 2 may inhibit apoptosis.

关 键 词：干燥综合征 泪腺 细胞凋亡 癌基因 基因表达 

分 类 号：R593.9[医药卫生—内科学]