灯盏细辛注射液对鼠实验性高眼压视神经轴浆运输的影响  

The affect of Erigeron Breviscapus (Vant.) Hand-Mazz on axoplasmic transport of optic nerve in rats with experimentally elevated intraocular pressure

作　　者：朱益华[1] 蒋幼芹[2] 刘忠浩[3] 罗学港[3] 吴振中[2] 

机构地区：[1]福建医科大学附属第一医院眼科 [2]湖南医科大学附属第二医院眼科,350005 [3]湖南医科大学神经生物学研究室

出　　处：《中华眼科杂志》2000年第4期289-291,I018,共4页Chinese Journal of Ophthalmology

摘　　要：目的　探讨灯盏细辛注射液是否对急性实验性高眼压大鼠视神经轴浆运输阻滞有促进恢复作用。方法　健康SD大鼠 30只 ,右眼制作成急性高眼压模型后 ,随机分成 3组。A组 6只鼠 ,做经左侧上丘逆行辣根过氧化物酶 (horseradishperoxidase ,HRP)标记 ,并行视网膜神经节细胞 (retinalganglioncells,RGCs)计数。B组 12只鼠 ,再分为灯盏细辛腹腔注射治疗组与对照组 ,每组各 6只鼠 ,高眼压 2 0d后做逆行标记RGCs计数。C组 12只鼠 ,再分为治疗组与对照组 ,每组各 6只鼠 ,高眼压 40d后做逆行标记RGCs计数。结果　A组视网膜未见标记的RGCs。B组治疗组及对照组标记的RGCs密度均数分别为 (749± 2 94)个 /mm2 和 (42 3± 2 2 0 )个 /mm2 ,两组间差异有显著性 (P <0 .0 1)。C组治疗组及对照组标记的RGCs密度均数分别为 (10 48± 393)个 /mm2 及 (6 10± 315 )个 /mm2 ,两组间差异有显著性 (P <0 .0 1)。结论　B组及C组治疗组的RGCs密度明显增高 。Objective To investigate whether Erigeron Breviscapus (Vant.) Hand Mazz (EBHM) can improve the optic nerve axoplasmic transport in rats with experimentally elevated intraocular pressure (IOP) Methods Thirty healthy SD rats were used for the study, acute elevated IOP model in the right eye was built, then they were divided into three groups randomly: Group A (0 day group) included six rats for retinal ganglion cell (RGC) counting via left superior colliculus retrograde horse radish perokidase labeling; Group B, twelve rats divided into EBHM treatment group and control group (6 rats in each subgroup) for RGC counting via left superior colliculus retrograde labeling after twenty days, and Group C included twelve rats submitted the same treatment and procedure as group B after 40 days Results After 0 day of acute elevated IOP, no labeled RGCs were observed After twenty days of acute elevated IOP, in the control and EBHM subgroups the density of labeled RGCs were (423±220)/mm 2 and (749±294)/mm 2 respectively, the difference between two subgroups showed statistical significance ( P <0 01) After 40 days of acute elevated IOP, the density of RGCs in the control and EBHM subgroups in group C were (610±315)/mm 2 and (1 048±393)/mm 2 respectively, the difference between the two subgroups being statistically significant ( P <0 01) Conclusion After 20 days and 40 days of acute elevation of IOP, the density of RGCs is obviously higher in EBHM group than that in the control group It is revealed that EBHM can improve the optic nerve axoplasmic transportation blocked by acute elevation of IOP in rats

关 键 词：灯盏细辛 大鼠 RGCS 高眼压 视神经轴浆运输 

分 类 号：R285[医药卫生—中药学]