人红细胞老化过程中花生凝集素和ConA受体电镜细胞化学研究  

AN ELECTRON MICROSCOPIC STUDY FOR CYTOCHEMICAL CHANGE OF CONCANAVALIN A RECEPTORS AND PEANUT AGGLUTININ RECEPTORS OF AGED HUMAN ERYTHROCYTES

作　　者：刘胜洪[1] 杨木兰 张春明 张亦农[1] 刘冰 官阳 郝刚 刘能保[1] 阮幼冰 

机构地区：[1]同济医科大学组织学胚胎学教研室 [2]超微病理学教研室 [3]老年医学教研室,武汉430030

出　　处：《解剖学报》2000年第3期268-270,I015,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金! ( 3 92 70 2 95 )

摘　　要：目的　观察人血液中 ,老化红细胞膜上花生凝集素 (PNA)和刀豆凝集素 (Con A)受体细胞化学反应的变化。　方法　应用金标花生凝集素 (PNAg)和刀豆凝集素 (Con Ag) ,对早期和老化红细胞膜上 PNA和 Con A受体进行电镜细胞化学显示 ,并用计算机图像分析系统对电镜照片进行定量处理。　结果　与早期红细胞相比 ,老化红细胞膜上 PNAg颗粒和 Con Ag颗粒均有明显增多。　结论　老化红细胞膜上唾液酸的丢失导致半乳糖基和甘露糖基暴露的相应增加 。Objective\ To observe the cytochemical changes of peanut agglutinin (PNA)receptors and concanavalin A(Con A)receptors of aged human erythrocytes. Methods\ Cytochemistry of Con A and PNA labelled with colloid gold (Con Ag and PNAg)on the surface of young and aged erythrocytes was observed under electron microscope,and the micrographs were processed by computer image analysis system. Results\ The number of Con Ag and PNAg particles of aged erythrocytes was significantly increased in comparison with that of young erythrocytes. Conclusion\ The desialylation of aged erythrocytes may result in increasing exposure of galactose and mannose,which may lead to the aged erythrocytes phagocytosed by macrophages.\;

关 键 词：红细胞 细胞老化 花生凝集素 细胞化学 CONA受体 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R362[医药卫生—基础医学]