大鼠三叉神经脊束核尾侧亚核-臂旁核-丘脑间接联系的研究  

STUDIES ON THE CAUDAL SPINAL TRIGEMINAL NUCLEUS- PARABRACHIAL NUCLEI-THALAMUS INDIRECT CONNECTION PATHWAY IN THE RAT

作　　者：陶发胜[1] 高蓉[1] 李云庆[1] 

机构地区：[1]第四军医大学解剖学教研室

出　　处：《解剖学报》2000年第2期108-112,I002,共6页Acta Anatomica Sinica

基　　金：国家杰出青年科学基金!( 3 962 5 0 11)

摘　　要：目的　观察臂旁核 (PBN)内向丘脑腹后内侧核 (VPM)投射神经元和三叉神经脊束核尾侧亚核 (Vc)向臂旁核投射纤维和终末的分布 ,以及两者之间的突触联系。　方法　 HRP逆行追踪与生物素葡聚糖胺 (BDA)顺行追踪相结合的双标技术 ,标记结果分别在光镜或电镜下观察。　结果　将 HRP注入 VPM后 ,在光镜下可见HRP逆标神经元主要位于同侧的臂旁外侧核、Kolliker- Fuse(KF)核和臂旁内侧核。将 BDA注入 Vc后 ,BDA顺标纤维和终末也主要见于同侧臂旁外侧核、KF核和臂旁内侧核 ,对侧丘脑 VPM、丘脑后核和丘脑胶状质核也有BDA顺标纤维和终末 ,但臂旁核内的 BDA顺标纤维和终末明显多于丘脑诸核团。在臂旁核内 ,HRP逆标神经元的主要分布区与 BDA顺标纤维和终末的主要分布区重叠。电镜下在臂旁核内可见 ,Vc向臂旁核投射的 BDA顺标纤维和终末与臂旁核向丘脑 VPM投射的 HRP逆标神经元形成以非对称性为主的轴 -树和轴 -体突触。　结论　大鼠中枢内存在 Vc- PBN- VPM间接联系通路 ,该通路在面口部躯体感觉信息 (包括伤害性信息 )的传递和调控过程中可能发挥重要作用。Objective\ To investigate:(1)distribution of neurons projecting from the parabrachial nuclei(PBN) to the thalamic ventral posteromedial nucleus(VPM) and the fibers and terminals originating from trigeminoparabrachial projection neurons;(2)synaptic connection between trigeminoparabrachial terminals and parabrachiothalamic projecting neurons. Methods\ Horseradish peroxidase(HRP) retrograde tracing combined with biotinylated dextran amine (BDA) anterograde tracing technique was used.The labeling results were observed under light of electron microscope,respectively. Results\ After injection HRP into the unilateral VPM,HRP retrogradely labeled nueronal cell bodies were mainly found in the ipsilateral lateral parabrachial nucleus(PBL),Klliker\|Fuse(KF) nucleus and medial parabrachial nucleus(PBM).After injection BDA into the unilateral Vc,BDA anterogradely labeled fibers and terminals were mainly encountered in the ipsilateral PBL,KF nucleus,PBM,and contralateral VPM,thalamic posterior nucleus and thalamic submedius nucleus.In the PBN,BDA anterogradely fibers and terminals were denser than that in the above mentioned thalamic nuclei.The main distribution area of HRP retrogradely labeled neurons overlapped with the BDA anterogradely labeled fibers and terminals within the PBN.Axo\|dendritic and axo\|somatic synapses between BDA anterogradely labeled terminals and dendrites or cell bodies of the HRP retrogradely labeled neurons within the PBN were observed under the electron microscope. Conclusion\ Vc\|PBN\|VPM pathway exists in the rat central nervous system and this indirect pathway might play important roles in the transmission and regulation of oro\|facial somatic sensory information,including noxious information.

关 键 词：臂旁核 丘脑腹后内侧核 VC 突触 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]