T-2毒素对低硒大鼠关节软骨抗氧化酶活性及基因表达的影响  

EFFECTS OF T-2 TOXIN ON ACTIVITIES AND GENE EXPRESSION OF ANTIOXIDANT ENZYMES IN SELENIUM DEFICIENT RAT’S ARTICULAR CARTILAGE

作　　者：薛森海[1] 王治伦[2] 陈静宏[2] 杨浩杰[2] 宋代琴[2] 赵长海[1] 

机构地区：[1]第四军医大学附属西京医院临床营养科,西安710032 [2]环境与疾病相关基因教育部重点实验室暨卫生部微量元素与地方病重点实验室,西安710061

出　　处：《营养学报》2013年第1期64-67,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金重点项目(No.30630058);陕西省自然科学基金(No.SJ08~ZT12);第四军医大学"2012年度精英人才系列资助计划"之"优秀文职人员培养基金"(4138C4IDK9)

摘　　要：目的研究T一2毒素对低硒（Se）大鼠关节软骨抗氧化酶及基因表达的影响。方法新生断乳雄性健康sD大鼠，平均体重60-80g，随机分成两大组即常规组和低se组喂养30d，低Se动物模型建造成功后再分成常规组、低se组、常规＋低T一2组、常规＋高T．2组、低se＋低T一2组、低se＋高T一2组，再T～2毒索灌胃饲养30d后，观察低se、T一2毒素及二者共同作用对各组大鼠关节软骨丙二醛（MDA）含量、总抗氧化能力（T—AOC）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH—Px）活性及SOD、CAT、GSH—PxmRNA表达的影响。结果与常规组相比，各组大鼠关节软骨中MDA含量升高，T—AOC、SOD、CAT和GSH—Px活性下降，均有统计学意义（P〈0．05）；低Se＋低T一2组、低se＋高T一2组与低se组相比，MDA含量升高，T—AoC、SOD、CAT和GSH—Px活性下降（P〈0．05）；与常规组相比，各组大鼠关节软骨SOD、CAT、GSH—PxmRNA表达降低（P〈0．05）。结论低se或单纯T一2毒素均可减弱大鼠关节软骨抗氧化酶活性，低se与T．2毒素对机体抗氧化功能有协同作用。Objective To study the effect of T-2 toxin on articular cartilage antioxidant enzymes and gene expression in selenium （Se） dificient rats. Method Weanling male SD rats （60-80） g were randomly divided into normal group and Se-deficient group and fed for 30 d. After the animal model of Se-deficiency was developed, the rats were randomly divided into 6 groups： normal group （ I ）, Se-deficient group （II）, normal＋low T-2 group （III）, normal＋high T-2 group （IV）, Se-deficient＋low T-2 group （V）, Se-deficient＋high T-2 group （VI）. The group I ,III, V were fed normal feed, and the other groups were fed low-Se feed. All groups were given T-2 toxin at 100 or 200 ng/（g bw＇d） except the group I and II. At the 30th day of the experiment, the content of malondialdehyde （MDA）, the total antioxidant capacity （T-AOC） and activities of superoxide dismutase （SOD）, catalase （CAT）, glutathione peroxidase（GSH-Px） and SOD, CAT, GSH-Px mRNA expression were observed. Results The MDA content in the other groups was significantly higher than group I （P〈0.05）. Besides, the T-AOC and activities of SOD, CAT, GSH-Px in the other groups were significantly lower than group I （P〈0.05）. Compared with group II, the content of MDA in group V and VI was increased and the T-AOC, activities of SOD, CAT, GSH-Px were decreased （P〈0.05） . Compared with group I, the mRNA expression of SOD, CAT, GSH-Px in the other groups were significantly lower （P〈0.05） . Conclusion Se-deficiency or T-2 toxin could weaken the activities of articular cartilage antioxidant enzymes. Se-deficiency and T-2 toxin had synergistic effect on the antioxidant function.

关 键 词：T-2毒素 硒 关节软骨 抗氧化酶 基因表达 

分 类 号：R114[医药卫生—卫生毒理学]