HPLC法测定大鼠血浆中穿心莲内酯类衍生物的含量及其药动学研究  

Determination of ADC in Plasma by HPLC and Its Pharmacokinetics in Rats

作　　者：刘蕾[1] 张瑜[1] 韩光[1] 华素[1] 

机构地区：[1]河南大学药物研究所,河南开封475004

出　　处：《中国药房》2013年第11期967-969,共3页China Pharmacy

基　　金：河南省科技创新人才项目资助(No.094100510020);河南大学校内自然基金资助项目(No.2009YBZR036)

摘　　要：目的:建立大鼠血浆中穿心莲内酯类衍生物(ADC)含量的高效液相色谱(HPLC)测定方法。方法:色谱柱为ODSC18(250mm×4.6mm,5μm),流动相为乙腈-水(23:77,V/V),流速为1.2ml/min,柱温为25℃,检测波长为208nm,进样量为20μl,内标物为芦荟苷。大鼠分别经灌胃和尾静脉注射给予ADC后,测定不同时间血药浓度。采用3p87软件计算药动学参数。结果:大鼠分别经灌胃和尾静脉注射给予ADC后,t1/2α分别为(1.47±0.15)、(0.45±0.03)h,t1/2β分别为(5.82±0.29)、(2.51±0.13)h,V/F分别为(1.32±0.12)、(0.38±0.04)L,AUC0-24h分别为(567.51±28.55)、(243.58±12.17)μg(/ml.h),CL/F分别为(0.53±0.03)、(0.16±0.02)L(/h.kg)。灌胃ADC后,tmax为2h,cmax为132.33μg/ml。结论:本方法操作简便、准确、灵敏度高、重复性好,可用于ADC血药浓度的监测及其药动学研究。To ADC in rat plasma and study the C18 column （250 mmx4.6 mm, 5 establish a high performance liquid chromatography （HPLC） method for the determination of pharmacokinetics of ADC in rats. METHODS： Chromatographic separation was performed on an pm） with acetonitrilewater （23 ： 77, V/V） as mobile phase at a flow rate of 1.2 ml/min with UV detection at 208 nm, the column temperature was at 25.C and aloin was used as an internal standard. ADC was intragastric administered and caudal vein injection to rats respectively .The concentrations of ADC in rat plasma were determined by HPLC and used to analyze the pharmacokinetic parameters in the compartment model by 3p87 program. RESULTS： After intragastric administration vs. caudal vein administration of ADC, pharmaceutical parameters were as follows ： t1/2αwere （ 1.47 ± 0.15 ） h, （0.45 ± 0.03 ） h; t1/2β were （5.82 ＋0.29）h,（2.51＋0.13）h; V/Fwere（1.32＋O.12）L,（0.38-＋O.O4）L; AUC0-24h were （567.51_＋ 28.35） μg/（ml · h） , （243.58 ± 12.17 ） pg/（ml· h ） ; CL/F were （ 0.53 ±0.03 ） L/（h. kg）, （ O. 16 ± 0.02 ） L/（ h. kg）. After intragastric administration of ADC, tmax was 2 h cmax was 132.33 pg/ml. CONCLUSION： The method is simple and accurate with a high sensitivity and a good repeatability, and it can be applied to the evaluation of pharmacokinetic parameters of ADC in rats and blood concentration of ADC in clinical controlling.

关 键 词：ADC 高效液相色谱法 药动学 

分 类 号：R285.5[医药卫生—中药学] R969.1[医药卫生—中医学]