魟鱼软骨多糖对小鼠黑色素移植瘤VEGF表达的影响  

Effect of RCG on the Expression of VEGF in Mice with Malignant Melanoma

作　　者：郭斌[1] 孙岩[2] 

机构地区：[1]辽宁医学院附属第一医院,辽宁锦州121000 [2]辽宁医学院,辽宁锦州121001

出　　处：《中国药房》2013年第11期970-972,共3页China Pharmacy

基　　金：辽宁省自然科学基金资助项目(No.201102132)

摘　　要：目的:研究魟鱼软骨多糖(RCG)对小鼠恶性黑色素移植瘤血管内皮生长因子(VEGF)表达的影响。方法:每只小鼠右腋皮下接种细胞以复制黑色素瘤模型,将实验分为模型(灌胃,等容生理盐水)、环磷酰胺(CTX,腹腔注射,60mg/kg)与RCG高、中、低剂量(灌胃,500、250、125mg/kg)组,CTX组每周给药1次,其余组每天给药1次,连续21d。观察肿瘤生长情况,计算瘤质量、抑瘤率;采用RT-PCR法和Western blot法检测黑色素瘤组织VEGF mRNA和蛋白表达。结果:与模型组比较,RCG高、中、低剂量组瘤质量显著减少,抑瘤率显著升高(P<0.01);RCG高、中、低剂量组VEGF mRNA和蛋白表达水平显著降低(P<0.01)。结论:RCG可明显抑制小鼠黑色素瘤原发瘤的生长,可能与其抑制肿瘤的VEGF mRNA表达和蛋白表达有关。To study the effect of ray cartilage glycosaminoglycans （.RCG） on the expression VEGF in mice with malignant melanoma. METHODS： Malignant melanoma model mice were induced by inoculating cell via subcutaneous tissue of their right armpit. The experimental mice were divided into model group（constant volume of normal saline）, CTX group（intraperitoneal administration, 60 mg/kg） and RCG high-dose, medium-dose and low-dose groups （gastric lavage administration, 500, 250, 125 mg/kg）. CTX group was given medicine once a week, and other group once a day, for 21 consecutive days.The growth of tumor was observed and the inhibitory rate of tumor was measured; the mRNA and the protein expression of VEGF were detected respectively with RT-PCR and Western blot. RESULTS： Compared with model group, inhibitory rates of primary tumor in RCG groups increased significantly, tumor weight decreased significatly （P〈0.01） ; the gene expression and the protein expression of VEGF in RCG groups lowered significantly（P〈0.01）. CONCLUSION： RCG could effectively inhibit the growth of malignant melanoma in mice, which was probably related to that RCG inhibit the gene expression of VEGF and the protein expression of VEGF.

关 键 词：魟鱼软骨多糖 恶性黑色素移植瘤 血管内皮生长因子 

分 类 号：R969.1[医药卫生—药理学] R971.1[医药卫生—药学]