芪苈强心胶囊对慢性心衰模型大鼠心肌纤维化及结缔组织生长因子表达的影响  

Effects of Qili Qiangxin Capsules on Myocardial Fibrosis and Expression of CTGF in Rats with Chronic Heart Failure

作　　者：张丽[1] 张睿[1] 周建中[1] 

机构地区：[1]重庆医科大学附属第一医院心内科,重庆400016

出　　处：《中国药房》2013年第11期975-978,共4页China Pharmacy

摘　　要：目的:研究芪苈强心胶囊对慢性心衰(CHF)模型大鼠心肌纤维化(MF)及结缔组织生长因子(CTGF)表达的影响。方法:对大鼠施以腹主动脉部分缩窄术复制CHF模型。实验分为假手术(等容生理盐水)、模型(等容生理盐水)、依那普利(10mg/kg)与芪苈强心胶囊高、中、低剂量(1、0.5、0.25mg/kg)组。灌胃给药,每天1次,连续4周。检测大鼠血流动力学参数,计算左室质量指数(LVMI),天狼猩红染色(PSR)观察左室心肌胶原形态,测量胶原容积分数(CVF),免疫组化法和Western blot法检测大鼠左室心肌组织中CTGF的表达。结果:与模型组比较,芪苈强心胶囊高、中、低剂量组LVMI、CVF和CTGF表达显著减弱(P<0.01或P<0.05);芪苈强心胶囊高、中剂量组LVEDP显著降低,-dp/dtmax显著升高(P<0.01或P<0.05),病理形态学显著改善,Ⅰ型/Ⅲ型显著降低(P<0.01);芪苈强心胶囊高剂量组+dp/dtmax显著升高(P<0.05)。结论:芪苈强心胶囊能减轻CHF模型大鼠的心室肥厚,抑制MF和改善心功能,该作用可能是通过抑制左室心肌组织中CTGF的过度表达来实现的。To explore the effects of Qili qiangxin capsule on myocardial fibrosis and expression of connective tissue growth factor （CTGF） in rats with chronic heart failure （CHF）. METHODS： CHF model was induced by partially banding abdominal aortic artery. Model rats were divided into sham-operation group （constant volume of normal saline）, model group （constant volume of normal saline）, enalapril group （10 mg/kg）, Qili qiangxin capsule high-dose, medium-dose and low-dose groups （1, 0.5, 0.25 mg/kg）. They were given medicine once a day for consecutive 4 weeks.Hemodynamic parameters and left ventricular weight index （LVMI） were detected, PicricSirius Red （PSR） Polarimetry was used to observe collagen morphology in left ventric ular interstitial tissue, measure collagen volume fraction （CVF） ; the expression of CTGF in left ventricular tissue was evaluated by immunohistochemical staining and Western blot analysis. RESULTS： Compared with model group, LVMI, CVF and CTGF protein expression of Qili qiangxin capsule high-dose, medium-dose and low-dose groups decreased significantly （P〈0.01 or P〈0.05）. LVEDP, I CVF/III CVF of high-dose and medium-dose groups decreased significantly（P〈0.01 ）;-p/dtmax increased significantly （P〈0.05） ; pathological morphology was improved.＋dp/dtmax of high-dose group increased significantly （P〈0.01 or P〈0.05）. CONCLUSIONS： Qiliqiangxin capsule attenuates ventricular hypertrophy in CHF rats, inhibits MF and improves cardiac function, which is dependent on its effect on exoressions of CTGF in left ventricular tissue.

关 键 词：芪苈强心胶囊 慢性心力衰竭 结缔组织生长因子 心肌纤维化 

分 类 号：R285[医药卫生—中药学] R97[医药卫生—中医学]