多元曲线分辨-交替最小二乘法应用于延胡索药材指纹图谱中重叠峰分辨及延胡索乙素的含量测定  

The Applications of Multivariate Curve Resolutional-ternating Least Squares in Resolving Overlapped Spectra of Corydalis yanhusuo Fingerprint and the Content Detection of Tetrahydropalmatine

作　　者：贾泽慧[1] 王春涛[1] 李华[2] 

机构地区：[1]太原师范学院化学系,太原030031 [2]西北大学分析科学研究所/中药现代化研究中心,西安710069

出　　处：《应用化学》2013年第3期329-334,共6页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(20975081);山西省自然科学基金(2011011007-2)资助项目

摘　　要：采用光谱相关色谱法对延胡索药材指纹图谱中的有效组分延胡索乙素峰进行确认;利用多元曲线分辨-交替最小二乘法(MCR-ALS)对延胡索乙素重叠峰簇进行解析,得到延胡索乙素的真实色谱峰,其光谱还原率大于0.999,并对其进行准确定量,该方法可作为指纹图谱的辅助方法,为中药的真伪鉴定和质量客观评价提供可靠依据;最后讨论了药材的炮制对延胡索乙素含量的影响。yanhusuo Spectral correlative chromatography was used to identify the peak of tetrahydropalmatine in Corydalis fingerprint. Moreover, multivariate curve resolution-alternating least squares （MCR-ALS） was used to resolve the overlapping peaks of tetrahydropalmatine and restore its true peak shape. More than 99.9% spectrum could be recovered and accurately quantitative results were obtained. The method can be used as an auxiliary fingerprinting method to provide a reliable basis for the authenticity and quality assessment of Chinese medicines. The effect of the herbs-cooking on the content of tetrahydropalmatine was also discussed.

关 键 词：延胡索药材 中药指纹图谱 光谱相关色谱 多元曲线分辨-交替最小二乘法 

分 类 号：O657.3[理学—分析化学]