银灵通胶囊减少人肝细胞系-5细胞的脂肪积聚作用及机制研究  

Yinlingtong capsules reduced the accumulation of fat in HHL-5 hepatocytes

作　　者：梁桂宁[1,2] 徐贞俊[1] 翁磊华[1] 侯亚义[2] 

机构地区：[1]南京大学医学院&南京大学国家生物医药技术重点实验室,210093 [2]广西医科大学生理学教研室,南宁530021

出　　处：《国际中医中药杂志》2013年第3期225-227,共3页International Journal of Traditional Chinese Medicine

摘　　要：目的观察银灵通胶囊对肝细胞系（HHL）．5细胞内甘油三酯含量、脂肪酸的氧化水平以及在脂肪氧化过程中起重要作用的代谢酶肉碱脂酰转移酶．1β（CPT-1β）、乙酰辅酶A羧化酶．[3（ACC-β）的影响。方法检测在不同剂量银灵通胶囊水溶物作用下人HHL-5细胞内甘油三酯含量、脂肪酸氧化水平，用Q-PCR检测银灵通处理HHL．5细胞中CPT-1β、ACC．B的mRNA水平。结果银灵通胶囊可降低HHL-5细胞内的甘油三酯含量，与对照组比较，银灵通胶囊溶液0．5、l、2mg／ml剂量组分别降低9．5％、14．3％、19．4％），HHL-5细胞脂肪酸氧化水平分别增加4.3％、6．9％和11．2％，并呈一定的剂量效应关系，但对CPT-1β、ACC．B没有影响。结论银灵通胶囊可通过增强HHL-5细胞脂肪酸氧化水平减少HHL-5细胞的脂肪积聚。Objective To study the effects of the Yinlingtong capsules on intracellular triglyceride, fatty acid oxidation levels and CPT-1β/ACC-β in HHL-5 cells. Methods After treated HHL-5 cells with different doses Yinlingtong capsules, intracellular triglyceride content and the fatty acid oxidation level were detected by the kits. The CPT-1β and ACC-β mRNA levels were detected by Q-PCR. Results Yinlingtong capsules could reduce triglyceride content （compared with the control group, triglyceride content of 0.5, 1 and 2 mg/ml dose group were reduced by 9.5%, 14.3% and 19.4%, respectively）, enhance the level of fatty acid oxidation by a dose-response relationship （compared with the control group, the level of fatty acid oxidation of 0.5, 1 and 2 mg/ml dose group were increased 4.3%, 6.9% and 11.2%, respectively）, but could not affect the CPT-1β and ACC-β mRNA levels. Conclusion Yinlingtong capsules reduced the accumulation of fat in HHL-5 hepatocytes, and the specific impact mechanism needs to be further explored.

关 键 词：银灵通胶囊 甘油三酯含量 脂肪酸氧化水平 肉碱脂酰转移酶-1β 乙酰辅酶A羧化酶-β 

分 类 号：R285.5[医药卫生—中药学]