肺炎克雷伯菌全基因组DNA芯片的研制和质量评价  

Development and evaluation of whole-genome DNA microarray of Klebsiella pneumoniae

作　　者：刘梦颖[1] 张义全[2] 王丽[1] 闫小娟 杨世亚[1] 周冬生[2] 杨瑞馥[2] 邱景富[1] 

机构地区：[1]重庆医科大学公共卫生与管理学院,重庆400016 [2]军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京100071

出　　处：《军事医学》2013年第2期122-125,共4页Military Medical Sciences

基　　金：国家自然科学基金资助项目(31071093,31170129);重庆市科委自然科学基金资助项目(2010BB5103)

摘　　要：目的研制肺炎克雷伯菌(KP)全基因组DNA芯片并对芯片质量进行评价。方法根据KP的NTUH-K2044全基因组序列,挑选出5075条基因,PCR扩增各基因并纯化产物,点样制备芯片。设计了3个质控杂交组合,采用双色荧光杂交策略,对芯片质量进行评价。结果与结论芯片杂交结果与理论预期结果一致。本研究成功研制了KP全基因组DNA芯片,并建立了基于全基因组DNA芯片的KP比较基因组学技术平台。Objective To develop and evaluate a whole-genome DNA mieroarray of Klebsiella pneumoniae. Methods Based on genomic sequences of K. pneumoniae NTUH-K2044, a total of 5075 genes were chosen, amplified by PCR and printed onto glass slides. Three sets of two-fluorescence comparative hybridizations were performed to evaluate the quality of the microarray. Results and Conclusion Microarray hybridization results were consistent with the anticipated results. The whole-genome DNA microarray of K. pneumoniae is successfully developed, and a method of microarray-based comparative genomic analysis of K. pneumoniae is established accordingly.

关 键 词：克雷伯菌 肺炎 DNA芯片 比较基因组学 

分 类 号：R346[医药卫生—基础医学]