Plackett-Burman设计联用星点设计-效应面法优化山药中二氧化硫残留量测定前处理工艺  

Optimization of Extraction Technology for Sulfur Dioxide from Dioscorea opposita by Plackett-Burman Design and Central Composite Design-response Surface Methodology

作　　者：彭月[1,2] 银玲[1,2] 李雪莲[1,2] 陈鸿平[1,2] 刘荣[1,2] 刘友平[1,2] 

机构地区：[1]成都中医药大学药学院,成都611137 [2]成都中医药大学中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137

出　　处：《中国实验方剂学杂志》2013年第5期32-35,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：四川省质量监督局重要技术标准研究项目(ZYBZ2011-50)

摘　　要：目的:运用Plackett-Burman试验设计和星点设计-效应面法优选山药中二氧化硫残留量测定前处理的最佳工艺。方法:以二氧化硫提取量为指标,采用单因素试验考察提取方法、溶剂用量、振荡速度、提取温度、提取时间及NaOH溶液质量分数对二氧化硫残留量测定前处理工艺的影响。运用Plackett-Burman试验设计筛选影响显著的因素,以NaOH溶液质量分数和溶剂用量为自变量,二氧化硫提取量为因变量,对自变量各水平进行多元线性回归和二项式拟合,通过效应面法选择工艺条件,并进行预测分析。结果:最佳前处理工艺为NaOH溶液质量分数0.7%,料液比1∶50.99,提取率实测值与预测值偏差3.23%。结论:优选的前处理工艺简便、预测性良好,为中药材中二氧化硫残留量测定时样品的前处理提供参考。Objective：To optimize extraction technology of sulfur dioxide from Dioscorea opposita by Plackett-Burman design and central composite design-response surface methodology. Method： With yield of sulfur dioxide as index,effect of extraction method,solvent amount,extraction temperature,extraction time and the mass fraction of NaOH solution on extraction technology of sulfur dioxide was investigated by single-factor test.Significant influencing factors were screened by Plackett-Burman design,independent variables were the concentration of NaOH and solvent amount,dependent variable was yield of sulfur dioxide,each level of independent variables was handled with multiple linear regression and binomial fitting,response surface methodology was used to optimize extraction process,and prediction was carried out through comparing the observed and predicted value. Result： Optimal extraction technology conditions were as followings：the mass fraction of NaOH 0.7%,solid-liquid ratio 1∶50.99, Bias of the observed value and the predicted value 3.23%. Conclusion： Optimized extraction technology was simple with good predictability,it could provide reference for sample pretreatment in determination of sulfur dioxide residues of traditional Chinese medicine.

关 键 词：山药 二氧化硫残留量 PLACKETT-BURMAN 星点设计 前处理 

分 类 号：R283.6[医药卫生—中药学]