分光光度法测定藏药翼首草不同药用部位总皂苷的含量  

Determination of Total Saponins from Different Medicinal Parts in Tibetan Medicine Pterocephalus hookeri by Spectrophotometry

作　　者：林升得[1] 江道峰[2] 张艺[1] 王宇[1] 谭尔[1] 

机构地区：[1]成都中医药大学民族医药学院,成都611137 [2]成都中医药大学药学院,成都611137

出　　处：《中国实验方剂学杂志》2013年第5期101-104,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81274193);四川省教育厅四川省省属高校科研创新团队建设计划项目(11TD004)

摘　　要：目的:测定藏药翼首草不同药用部位总皂苷的含量,并比较其不同部位之间的差异。方法:以熊果酸为对照,用5%的香草醛-冰醋酸及高氯酸进行显色,在540 nm波长下运用紫外分光光度法对翼首草不同部位总皂苷含量进行测定。结果:在0.058 2～0.203 8 mg熊果酸的吸光度与含量线性关系良好(r=0.999 1)。平均回收率97.06%,RSD 1.93%。结论:翼首草地上部分总皂苷平均含量高达5%,结合目前藏医院用药情况及药理研究现状,在保护资源的前提下,同时又不影响其疗效的情况下,建议以地上部分为其入药部位。Objective：To determine the total saponins from P. hookeri in different medicinal part and compare the differences in the different parts. Method： Ursolic acid was selected as control article. Coloration was used with 5% vanillin and perchloric acid. The content of total saponins from P. hookeri in different medicinal parts was determined at 550 nm by UV. Result： Ursolic acid was linear within the range of 0.058 2-0.203 8 mg; the correlation coefficient r=0.999 1. The average recovery was 97.06%, RSD 93%. Conclusion： The average content of total saponins from aerial parts was up to 5%. Combining Tibetan hospital drug use and pharmacological research status, in the premise of resource protection, and do not affect its curative effect at the same time, it is recommended that the aerial part of P. hookeri should be its medicinal part.

关 键 词：藏药 翼首草 总皂苷 不同药用部位 分光光度法 

分 类 号：R284.1[医药卫生—中药学]