HPLC波长切换法同时测定枳芍散水煎液中4个成分的含量  

Determination of Four Components in Decoction of Zhishao Powders by HPLC with UV Switch

作　　者：陈家仪[1] 贾薇[1] 曾元儿[1] 陈稚[1] 江滨[1] 

机构地区：[1]广州中医药大学,广州510006

出　　处：《中国实验方剂学杂志》2013年第5期114-116,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省大学生创新实验项目(1057211030)

摘　　要：目的:建立高效液相色谱波长切换法对枳芍散水煎液中4个成分(芍药苷、柚皮苷、橙皮苷、新橙皮苷)进行分析。方法:采用Syncronis C18(4.6 mm×250 mm,5μm)色谱柱,以0.3%磷酸水溶液(A)-乙腈(B)为流动相,二元梯度洗脱,流速1.0 mL.min-1,检测波长为230 nm(0～30 min测定芍药苷)、283 nm(30～60 min测定柚皮苷、橙皮苷、新橙皮苷)。结果:枳芍散中4个成分芍药苷、柚皮苷、橙皮苷、新橙皮苷进样量分别在0.12～1.73μg(r=0.999 6),0.38～5.68μg(r=0.999 9),0.64×10-1～0.96μg(r=0.999 9),0.72～10.83μg(r=0.999 7)呈良好线性关系;平均回收率分别为104.17%,100.98%,103.72%,101.01%。结论:该方法准确可靠、重复性好,可用于枳芍散的质量控制。Objective：To develop a HPLC method for determination of four indicative components （paconiflorin, naringin, hesperidin and neohesperidin） in decoction of Zhishao powders. Method： Syncronis C18 （4.6 mm×250 mm,5 μm） was adopted; and the mobile phase was 0.3% phosphoric acid solution （A）-acetonitrile （B） with gradient elution at a flow rate of 1.0 mL·min-1, and the detection wavelength was 230 nm （0-30 min） for paconiflorin, 283 nm （30-60 min） for naringin, hesperidin, neohesperidin. Result： The content of five indicative components in decoction of Zhishao powders was stable. The method had a good linearity in the ranges of 0.12-1.73 μg （r=0.999 6） for paconiflorin, 0.38-5.68 μg （r=0.999 9） for naringin, 0.64×10-1-0.96 μg （r=0.999 9） for hesperidin, 0.72-10.83 μg （r=0.999 7） for neohesperidin. The average recoveries was 104.17%, 100.98%, 103.72% and 101.01% respectively. Conclusion： This method is dependable, simple and practical, and may be use to control the quality of Zhishao powders.

关 键 词：枳芍散 波长切换 含量测定 

分 类 号：R284.1[医药卫生—中药学]