HPLC-DAD同时测定鸡肉参中芦丁、槲皮苷、槲皮素的含量  

Simultaneous Determination of Rutin,Quercetin and Quercitroside in Incarvillea mairei by HPLC-DAD

作　　者：杨月娥[1] 朱恩焕[1] 胡文[1] 袁云[1] 马兴芬[1] 刘德举[1] 

机构地区：[1]大理学院药学院,云南大理671000

出　　处：《中国实验方剂学杂志》2013年第5期120-122,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：大理市科技局科研基金资助项目(2008SF11)

摘　　要：目的:建立同时测定鸡肉参中芦丁、槲皮苷、槲皮素含量的HPLC-DAD方法。方法:采用Diamonsil-C18色谱柱(150 mm×4.6 mm,5.0μm),柱温30℃,以甲醇(A)-0.5%磷酸水溶液(B)为流动相,进行梯度洗脱(0～5 min,48%～52%A;6～10 min,70%～30%A),检测波长254 nm,流速1.0 mL.min-1。结果:3种黄酮类成分在10 min内达到良好分离,芦丁、槲皮苷、槲皮素的线性范围分别为0.3～1.0 mg(r=0.999 8),0.030～0.10 mg(r=0.999 8),0.010～0.033 mg(r=0.999 5);平均加样回收率分别为100.95%(RSD 0.98%),99.51%(RSD 1.54%),102.84%(RSD 3.53%)。结论:该方法简便,快速,精确,可同时测定鸡肉参中芦丁、槲皮苷、槲皮素的含量,具有较好的重复性和稳定性,可作为鸡肉参药材的质量控制方法。Objective：To establish an HPLC-DAD method for simultaneous determination of rutin, quercetin and quercitroside in Incarvillea mairei. Method： A Dikma Diamonsil C18column （4.6 mm×150 mm, 5 μm） was used. The column temperature was set at 30 ℃; the investigated compounds were separated with gradient mobile phase consisting of methanol （A）-0.5% phosphoric acid （B）; the flow rate was 1.0 mL·min-1 and the detection wavelength was kept at 254 nm. Result： The good separation of three flavonoids was achieved within 10 min. The linearity was achieved in the range of 0.30-1.0 mg （r=0.999 8） for rutin, 0.030-0.10 mg （r=0.999 8） for quercetin and 0.010-0.033 mg （r=0.999 5） for quercitrosid. The average recoveries were 100.95% （RSD 0.98%）, 99.51% （RSD 1.54%） and 102.84% （RSD 3.53%） respectively. Conclusion： The method is simple, rapid and accurate, which can be able to detect rutin, quercetin and quercitroside simultaneously, and can be used for quality control of I. mairei.

关 键 词：HPLC 鸡肉参 芦丁 槲皮苷 槲皮素 

分 类 号：R284.1[医药卫生—中药学]