大黄游离蒽醌对犬重症急性胰腺炎早期肠损伤的保护作用  

Protective Effect of Rhubarb Free Anthraquinone on Intestinal Barrier Injury in Beagle Dogs Induced by Severe Acute Pancreatitis

作　　者：张燕[1] 王平[1] 杨永茂[1] 张艳[1] 于宜平[1] 孟宪丽[1] 陈小睿[1] 

机构地区：[1]成都中医药大学药学院,成都611137

出　　处：《中国实验方剂学杂志》2013年第5期172-176,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81073118);四川省教育厅自然基金重点项目(11ZA061)

摘　　要：目的:探讨单次灌胃大黄游离蒽醌对重症急性胰腺炎性肠损伤的保护作用。方法:采用逆行胰管注入5%牛磺胆酸钠制备犬重症急性胰腺炎模型,将动物随机分为假手术组、模型组、给药组,造模后6 h给药组动物灌胃大黄总游离蒽醌20 mg.kg-1,手术前、给药前、给药后不同时间点取血测定外周血液中血清淀粉酶活性、肿瘤坏死因子-α(TNF-α)含量和内毒素含量变化,给药前、给药后不同时间点取血测定门静脉中二胺氧化酶活性变化。结果:给药组与模型组相比,给药后0到6 h血清淀粉酶活性无明显降低,外周血中α肿瘤坏死因子(TNF-α)水平在给药后5 h显著降低(P<0.05),门静脉血中二胺氧化酶活性显著降低(P<0.05),但外周血液中内毒素含量反而显著升高(P<0.05)。结论:大黄游离蒽醌可通过减轻机体炎症反应和肠上皮细胞损伤、改善肠道微循环,来发挥对重症急性胰腺炎性肠损伤的保护作用。Objective：To study the protective effect of rhubarb on intestinal barrier injury of dogs with severe acute pancreatitis. Method： Fifteen healthy Beagel dogs were used to establish severe acute pancreatitis model by retrograde pancreatic duct injection and were randomly divided into sham group,model group and treatment group. The treatment group was given rhubarb anthraquinones of total extracted by stomach injection at 6 hours after modeling. Result： The contents of endotoxin in peripheral blood was less than sham operation group, at 7,11 hour after modeling was significantly lower （P〈0.05）, after administration the level of endotoxin had the tendency of increase and 3 hours after drug administration it was significantly higher than the model group （P〈0.05）. In early times of severe acute pancreatitis, peripheral blood amylase and tumor necrosis factor（TNF-α） was significantly increased, while the level of endotoxin was lower. Rhubarb anthraquinone had not significant affect on the serum amylase of SAP, it could inhibit the sustained increaseof peripheral blood TNF-α levels and increase in peripheral blood plasma levels of endotoxin in early time of SAP. Conclusion： The effect of Rhubarb on severe acute pancreatitis with endotoxin translocation in early time may be ralated to that it can inhibit the inflammatory response improve intestinal microcirculation and restore normal intestinal absorption.

关 键 词：大黄游离蒽醌 重症急性胰腺炎 肠屏障 内毒素 

分 类 号：R285.5[医药卫生—中药学]