母婴间HLA-Cw基因多态性与子痫前期的关联性研究  

Study on correlation between gene polymorphism of maternal-fetal HLA-Cw and preeclampsia

作　　者：张琳琳[1] 贾莉婷[1] 张展[1] 

机构地区：[1]郑州大学第三附属医院检验科,河南郑州450052

出　　处：《中国妇幼保健》2013年第7期1154-1157,共4页Maternal and Child Health Care of China

基　　金：国家自然科学基金项目〔30872316〕;河南省医学科技攻关项目〔2011020058〕;河南省重点科技攻关计划〔112102310089〕

摘　　要：目的:探讨母婴间HLA-Cw基因多态性与子痫前期的相关性。方法:采集117例子痫前期患者和106例正常孕妇外周血及其胎儿脐带血,提取DNA后采用PCR-SSP方法测定孕妇及胎儿的HLA-Cw基因型进行统计分析。结果:子痫前期组孕妇HLA-Cw12的频率(19/234)要显著高于正常孕妇组孕妇(7/212),子痫前期组母儿共携带HLA-Cw12基因的频率(13/117)显著高于正常晚孕组(4/106),差异均有统计学意义(P<0.05)。HLA-Cw按照与KIR受体结合的类型分为C1和C2组,但无论在孕妇还是新生儿中C1和C2型等位基因在子痫前期组和正常晚孕组间的分布差异均无统计学意义(P>0.05)。结论:HLA-Cw12的基因多态性可能与子痫前期的发病相关。Objective： To explore the correlation between gene polymorphism of maternal - fetal human leukocyte antigen - Cw （ HLA - Cw） and preeclampsia. Methods： The peripheral blood samples of 117 patients with preeclampsia and 106 normal pregnant women and umbilical cord samples of their fetuses were collected, PCR - SSP method was used to statistically analyze HLA - Cw genotypes of preg- nant women and their fetuses after abstracting DNA. Results： The frequency of HLA - Cwl2 in pregnant women in preeclampsia group （ 19/ 234 ） was statistically significantly higher than that in normal pregnancy group （7/212） , the frequencies of HLA - Cwl2 in pregnant women and their fetuses in preeclampsia group （ 13/117 ） were statistically significantly higher than those in normal pregnancy group （4/106） （ P 〈 0. 05 ） . HLA - Cw was divided into C1 group and C2 group according to their types of combination with KIR receptor, but there was no sta- tistically significant difference in distributions of C1 and C2 alleles in pregnant women and their neonates between preeclampsia group and normal late pregnancy group （P 〉 0. 05 ） . Conclusion： HLA- Cw12 gene polymorphism may be correlated with onset of preeclampsia.

关 键 词：人类白细胞抗原 子痫前期 基因多态性 

分 类 号：R714.245[医药卫生—妇产科学]