二氧化氯灭活水中肠道腺病毒及其分子机制研究  

Mechanism and regularity of inactivation of adenovirus by chlorine dioxide in water

作　　者：刘艳芝[1,2] 薛斌[2] 陈照立[2] 王新为[2] 郭旋[2] 金敏[2] 李君文[2] 

机构地区：[1]中南大学公共卫生学院,湖南长沙410000 [2]军事医学科学院卫生学环境医学研究所天津市环境与食品安全风险监控技术重点实验室

出　　处：《环境与健康杂志》2013年第2期95-98,共4页Journal of Environment and Health

基　　金：国家自然科学基金重点项目(30930078)

摘　　要：目的探讨二氧化氯对肠道腺病毒41型(AdV41)核酸的破坏在灭活病毒中的作用,并对病毒基因组受损区域进行定位,阐明二氧化氯灭活腺病毒的消毒规律和机制。方法在25℃,pH 7.2条件下,以不同剂量(0.1、0.5、0.7、1.0、2.0 mg/L)的二氧化氯对AdV41作用不同时间(0、0.5、1、2、3、4、5、7、10、20、30、60 min);采用大片段逐步步移PCR(long-overlapping PCR)分析二氧化氯对病毒全基因组的损伤,以细胞培养法检测病毒感染性。结果二氧化氯浓度变化符合幂函数Ct=a0×e-kt规律;病毒的灭活率随着Ct值的增加而逐渐增高;当Ct=0.773 mg(/L.min)时,二氧化氯对AdV41可以达到4log的灭活对数值(即灭活率为99.99%)。AdV41基因组各部分对二氧化氯的抵抗力不同,其5′的1～2 081 nt区域的破坏与AdV41感染性的消失一致。结论 AdV41基因组5′的1～2 081 nt区域对二氧化氯最为敏感,且与病毒的感染性相关;在25℃,pH 7.2时二氧化氯灭活4log AdV41的Ct值为0.773 mg(/L.min)。Objective To evaluate the role of the destruction of viral DNA in the inactivation of adenovirus 41 by chlorine dioxide, and locate the damaged sites in viral genome, so as to provide the basis for elucidating the inactivation regularity and mechanism of adenovirus 41 by chlorine dioxide. Methods Long-overlapping polymerase chain reaction （PCR） was used to detect the damage of viral genome, and cell culture was applied to determine viral infectivity at different chlorine dioxide dosages （0.1,0.5,0.7,1.0 and 2.0 mg/L） for different contact time（0,0.5,1,2,3,4,5,7,10,20,30 and 60 rain）. Results The concentration of chlorine dioxide was consistent with the power function Ct=a0×e^-1a. The inactivation rate of virus was increased with the Ct values,being a dose-effect relationship. Ct values for 4 log inactivation at 25 ℃ was 0.773 mg/（L·min） for pH7.2. Different regions of AdV41 viral genome had different chlorine dioxide-resistance. The damage of the 1-2 081 nt of the genomic 5＇regions was in coincidence with the inactivation of AdV41. Conclusion Damage of the 1-2 081 nt of the genomic 5＇regions may cause lethal effect by chlorine dioxide. Adenovirus 41 Ct values for 4 log inactivation at 25 ℃ was 0.773 mg/（L·min） for pH7.2.

关 键 词：水消毒 腺病毒 二氧化氯 大片段逐步步移PCR 

分 类 号：R123.6[医药卫生—环境卫生学]