镉与汞联合作用对人胚肝细胞凋亡和DNA损伤的影响  

Joint effects of cadmium and mercury on DNA damage and apoptosis in human embryo hepatocytes

作　　者：蓝晖翔[1] 江松[1] 纪存委[1] 郑丹[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院国家环境保护总局环境与健康重点实验室,湖北武汉430030

出　　处：《环境与健康杂志》2013年第2期98-101,共4页Journal of Environment and Health

基　　金：教育部中央高校基本科研业务费专项资金(HUST:2011QN204)

摘　　要：目的研究镉与汞单独及联合染毒对人胚肝细胞(L02细胞)凋亡及DNA损伤的作用。方法以0.01～100μmol/L的氯化镉、氯化汞以及等浓度的氯化镉、氯化汞混合液对L02细胞染毒24 h,采用MTT法测定细胞的存活率,根据Finney法判断镉与汞对L02细胞的联合作用类型;采用单细胞凝胶电泳技术(SCGE)和流式细胞术(FCM)检测L02细胞的DNA损伤和细胞凋亡情况。结果 0.01μmol/L的氯化镉、氯化汞单独及联合染毒可以刺激细胞的生长,但≥1μmol/L的氯化镉、氯化汞单独及联合染毒可显著抑制细胞的生长;且联合作用表现为相加作用。镉、汞单独及联合作用可致L02细胞的DNA损伤率和细胞凋亡率均显著高于对照组;且随着染毒浓度的升高,L02细胞的DNA损伤率和细胞凋亡率均呈上升趋势。结论镉汞联合染毒引起的DNA损伤和细胞凋亡可能存在一定的协同效应,这可能是由于镉、汞诱导细胞发生氧化应激所致。Objective To study the effects of cadmium and mercury alone and in combination on DNA damage and apoptosis in human embryo hepatocytes （L02 cells）. Methods L02 cells were exposed to 0.01-100 μmol/L of cadmium chloride （CdC12）,mercury chloride （HgC12） and their mixtures（CdCl2＋HgCl2） for 24 hours. MTT method was used to measure the survival of L02 cells,and then the joint effects of these mixtures were judged depending on the Finney law. Single cell gel electrophoresis （SCGE） and flow cytometry （FCM） were used to measure the DNA damage and apoptosis of L02 cells respectively. Results 0.01 μmol/L of cadmium,mercury and their mixtures could stimulate the growth of L02 cells,but when the concentration of cadmium, mercury and their mixtures were more than 1μmol/L,they could significantly inhibit the growth of L02 cells, and the joint effects of cadmium and mercury were additive action. The rates of DNA damage and the percentages of apoptosis were significantly higher than the control group after exposure to cadmium,mercury and their mixtures for 24 hours,and the rate of DNA damage and the percentage of apoptosis showed an ascendant trend with the increase of individual dose. Conclusion The joint exposure of cadmium and mercury may has synergistic toxic effect, which may be due to the oxidative stress induced by cadmium and mercury.

关 键 词：镉 汞 人胚肝细胞 细胞凋亡 DNA损伤 

分 类 号：R994.6[医药卫生—毒理学]