纳米二氧化钛对大鼠脑皮层组织结构及其氧化应激水平的影响  

Effects of Nano-TiO_2 on cerebral cortex structure and oxidative stress in rats

作　　者：王文斌[1] 李国珍[1] 张凤梅[1] 于丽华[2] 李仓廪[1] 袁华[1] 鄂蒙[1] 赵慧男[1] 李杰[1] 

机构地区：[1]山东大学公共卫生学院环境与健康系,山东济南250012 [2]山东大学公共卫生学院实验室管理中心

出　　处：《环境与健康杂志》2013年第2期113-116,F0003,共5页Journal of Environment and Health

基　　金：山东省自然科学基金青年基金(ZR2010HQ023);山东大学自主创新基金自然科学类专项(2010TS033);山东大学公共卫生学院青年人才创新基金(201001)

摘　　要：目的研究纳米二氧化钛对大鼠脑皮层组织结构及其氧化应激水平的影响。方法将50只健康SPF级雄性Wistar大鼠随机分为5组,分别为对照(蒸馏水)组和低(62.5 mg/kg)、中(125 mg/kg)、高(250 mg/kg)剂量纳米二氧化钛染毒组及普通粒径二氧化钛染毒(250 mg/kg)组,每组10只。采用灌胃方式进行染毒,每天1次,连续染毒60 d。测定大鼠脑皮层总超氧化物歧化酶(T-SOD)活力、谷胱甘肽过氧化物酶(GSH-Px)活力、总抗氧化能力(T-AOC)和丙二醛(MDA)含量,并观察大脑皮层组织结构改变。结果与对照组比较,高剂量纳米二氧化钛染毒组大鼠脑皮层T-SOD活力下降,中、高剂量纳米二氧化钛染毒组大鼠脑皮层MDA含量升高,差异均有统计学意义(P<0.05);各剂量纳米二氧化钛染毒组大鼠脑皮层GSH-Px活力和T-AOC均无显著变化。普通粒径二氧化钛染毒组大鼠以上4个指标与对照组比较,差异均无统计学意义。纳米二氧化钛染毒大鼠皮层神经细胞出现细胞肿胀、核固缩、核碎裂、染色质溶解等现象;普通粒径二氧化钛组组织结构基本正常,仅出现轻度核固缩及染色质溶解现象。结论在本实验剂量下,纳米二氧化钛染毒可导致大鼠脑皮层组织结构损伤和过氧化水平升高。Objective To study the influence of nano-titanium dioxide on the cerebral cortex structure and level of oxidative stress in rats. Methods A total of 50 SPF Wistar rats were randomly divided into five groups：control group（distilled water） , three nano-titanium dioxide groups （62.5,125 and 250 mg/kg body weight） and ordinary particle size group （250 mg/kg body weight）. Titanium dioxide was given to rats by oral garage, once a day for 60 consecutive days.The levels of T-SOD, MDA,GSH-Px and T-AOC in cortex were determined. The cerebral cortex pathological slices were prepared to observe the structural changes of the cerebral cortex. Results Compared with the control group, the level of T-SOD decreased significantly in 250 mg/kg group （P〈0.05）, the level of MDA increased significantly in 250 mg/kg group and 125 mg/kg group （P〈0.05）. Pathological examination showed cell swelling,nuclear condensation,fragmentation and chromatolysis in nano-titanium exposed groups. No significant damages were observed in the cerebral cortex in ordinary particle size group. Conclusion Nano- titanium dioxide exposure may cause damage of cerebral cortex tissues and increase the level of peroxide in rats.

关 键 词：纳米二氧化钛 皮层 氧化应激 

分 类 号：R994.6[医药卫生—毒理学]