铁皮石斛无性系繁育培养基专用性的研究  

Special culture mediums of breeding clones of Dendrobium officinale

作　　者：章晓玲[1] 张玲菊 朱玉球[1] 王林华 

机构地区：[1]浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江省铁皮石斛产业技术创新战略联盟,浙江临安311300 [2]宁波市农业科学研究院,浙江宁波315000 [3]浙江佳诚生物工程有限公司,浙江义乌322002

出　　处：《中国中药杂志》2013年第4期494-497,共4页China Journal of Chinese Materia Medica

基　　金：浙江省重大科技专项(2012C12912-9)

摘　　要：以浙江雁荡山×云南广南种质的杂交组合茎段为外植体,筛选出1/2MS+IBA 1.0 mg.L-1+6-BA 1.0 mg.L-1腋芽诱导培养基与1/2MS+IBA 1.5 mg.L-1+6-BA 0.5 mg.L-1芽增殖培养基,在浙江、云南、广西、湖南等159个铁皮石斛野生与人工种质中应用,腋芽诱导率达94.3%,具有较好的通用性;芽增殖培养时,不同种质分化出单芽、丛芽、原球茎3种增殖途径,增殖效果存在显著差异。研究结果表明,不同种质铁皮石斛的增殖对培养基有专用性需求,开发铁皮石斛无性系繁育专用培养基具有紧迫性和重要的应用价值。The explants were obtained from stem segments of hybrid combination of Dendrobium offwinale germplasms （Zhejiang Yandang Mountain x Yunnan Guangnan）. The screened culture mediums for buds induction and multiplication, which were 1/2MS ＋IBA 1.0 mg·L-1 ＋6-BA 1.0 mg ·L-1 and 1/2MS ＋IBA 1.5 mg ·L-1 ＋6-BA 0. 5 mg·L-1 respectively, were applied to 159 germplasms of D. officinale from Zhejiang, Yunnan, Guangxi, Hunan, etc. The medium for axillary buds induction had universality with 94. 3% induction rate. During buds multiplication, there were significant differences in proliferation effect among germplasms, and three proliferation forms i.e. single bud, multiple buds and protocorm were differentiated from different germplasms. The results showed that different germplasms of D. officinale had specific requirements for culture medium. Therefore, developing special culture medium for breeding clones of D. officinale is urgent and has important application values.

关 键 词：铁皮石斛 组织培养 无性系 专用培养基 

分 类 号：S567.239[农业科学—中草药栽培]