调补肺肾三法对慢性阻塞性肺疾病大鼠右心室重构的影响和远后效应  

Short and Long Term Effects of Three Methods of Regulating and Supplementing the Lung and Kidney on the Right Ventricular Remodeling in Rats with Chronic Obstructive Pulmonary Disease

作　　者：王英[1] 李亚[2] 李建生[1,2] 李素云[2] 田燕歌[1] 姜素丽[1] 邓丽[1] 王元元[2] 

机构地区：[1]河南中医学院老年医学研究所,河南省郑州市金水路1号450008 [2]河南中医学院第一附属医院呼吸病研究所

出　　处：《中医杂志》2013年第5期415-419,429,共6页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81130062);2010年度河南省科技创新人才项目(104200510004)

摘　　要：目的评价调补肺肾三法对慢性阻塞性肺疾病(COPD)大鼠右心室重构的影响和远后效应。方法 120只大鼠随机分为对照组、模型组、补肺健脾组、补肺益肾组、益气滋肾组和氨茶碱组,每组20只。除对照组外其余各组采用香烟暴露联合细菌感染法制作COPD模型8周,于第9周起分别给予补肺健脾方、补肺益肾方、益气滋肾方及氨茶碱灌胃至第20周。于第20、32周分批取材,观察大鼠心肌组织超微结构,计算右心肥大指数(RVHI)和心肌组织细胞因子的表达。结果第20、32周时,各给药组及对照组大鼠RVHI均低于模型组(P<0.01);补肺健脾组、补肺益肾组、益气滋肾组大鼠RVHI较氨茶碱组显著降低(P<0.05或P<0.01)。第20、32周时,模型组大鼠肌节长度较对照组缩短(P<0.05),补肺健脾组较模型组增长(P<0.01)。第20、32周时,模型组大鼠心脏组织细胞因子表达高于其余各组(P<0.01);补肺健脾组、补肺益肾组、益气滋肾组大鼠内皮素-1(ET-1)表达较氨茶碱组明显降低(P<0.01)。结论调补肺肾三法可改善COPD右心室重构并有明显远后效应,其中以补肺健脾方和补肺益肾方尤为显著,其作用机制可能与调节细胞因子有关。Objective To evaluate the short and long term effects of three methods of regulating and supplementing the lung and kidney on the right ventricular remodeling （RVR） in rats with chronic obstructive pulmonary disease （COPD）. Methods Totally 120 rats were randomized into control group, model group, lung-supplementing spleen-fortifying group, lung-supplementing kidney-boosting group, qi-boosting kidney-enriching group and aminophylline group, with 20 in each. The COPD models were established with cigarette smoke exposure and bacterial infection in the five groups except the control group. After 8 weeks of modeling, each group was intragastrically administered with the formulas of lung-supplementing spleen-fortifying, lung-supplementing kidney-boosting, qi-boosting kidney-enriching and aminophylline respectively from the 9th week to the 20th week. The rats were sacrificed and the myocardial tissue samples were obtained after 20 or 32 weeks of treatment. The ultrastructure of myocardial tissue was observed. The right ventricular hypertrophy index （RVHI） was calculated and the myocardi- al tissue factor expression was observed. Results Comparing with the model group, the RVHI was significantly decreased in other groups after 20 or 32 weeks of treatment （P〈0.01）. Comparing with the aminophylline group, the RVHI was signifi- cantly decreased in the lung supplementing spleen fortifying group, lung supplementing kidney boosting group and qi boosting kidney enriching group （P〈0.05 or P〈0.01）. The sarcomere length in the model group was shorter than that in the control group after 20 or 32 weeks of treatment （P〈0. 05）. The sarcomere length in the lung supplementing spleen fortifying group was longer than that in the model group after 20 or 32 weeks of treatment （P〈0.01）. The myocardial tissue factor expression in the model group was significantly higher than those in other groups after 20 or 32 weeks of treatment （P〈0.01）. Comparing with the aminophylllne group, the endothelln-I �

关 键 词：补肺健脾 补肺益肾 益气滋肾 慢性阻塞性肺疾病 右心室重构 远后效应 

分 类 号：R285.5[医药卫生—中药学]