江苏省男性HIV感染者梨支原体16SrRNA基因片段序列分析  

Analysis on Mycoplasma pirum infection in male HIV/AIDS patients and related 16S rRNA genes in Jiangsu province

作　　者：吴建茹[1] 朱一[1] 谢彦昕[1] 周良佳[1] 徐金水[2] 还锡萍[2] 王蓓[1] 

机构地区：[1]东南大学公共卫生学院流行病与卫生统计学系,南京210009 [2]江苏省疾病预防控制中心

出　　处：《中华流行病学杂志》2013年第3期259-262,共4页Chinese Journal of Epidemiology

基　　金：国家自然科学基金（30872156）

摘　　要：目的了解梨支原体（怖i）在男性HIV感染者中的流行情况，复核鉴定部分脚i16SrRNA基因片段序列。方法以江苏省男性HIV／AIDS为研究对象，采集其首段尿，用巢式PCR法检测ipi，并描述该人群中且如i的感染情况。纯化PCR产物进行Mpi16SrRNA基因片段序列测定，采用Mega4．0软件的多序列排列程序对支原体临床株和下载序列进行同源位排列、比对，利用Neighbor-Joining构建系统发育树。将Mpi的p1基因序列信息输入VectorNTIAdvance11．0软件进行分析，并推算其编码的氨基酸序列，将推断出的理论氨基酸序列与其他支原体p1全序列进行同源性分析。结果Mpi总感染率为21．5％。18株临床分离株几乎全部汇聚为一个分支，相似度〉90％，且与实验室保存Mpi标准株在同一分支，Mpi的p1蛋白与穿通支原体HF-2同源关系较近。结论ipi的感染率与以往调查结果相比明显增高，且临床分离株具有较高同源性。Objective To investigate the prevalence ofMycoplasma pirum （Mpi） in male HIV infected patients, and to identify the 16S rRNA gene of Mpi. Methods The first void urine of male HIV/AIDS patients in Jiangsu province was collected for Mpi detection. Purified 16S rRNA gene PCR production was sequenced for analysis on its identification, homogeneity and phylogenetic tree. P1 protein sequence of Mpi was analyzed by Vector NTI Advance 11.0 to calculate the coded amino acid sequence. Homogeneity analysis was conducted between the theoretical amino acid sequence of Mpi and other Mycoplasmas. Results The prevalence of Mpi in male HIV/AIDS patients was 21.5% while the Mpi prevalence rates in different age groups were significantly different （X2= 124.63, P〈 0.01 ）. The homogeneity of 18 strains of Mpi was higher than 90%. Conclusion The Mpi prevalence seemed much higher than the results from previous detection on HIV/AIDS patients, suggesting that more attention should be paid on AIDS treatment. More bioinformatic research on gene/nucleotide sequence analysis and forecast should be carried out to identify the molecular characteristics of Mpi.

关 键 词：梨支原体 HIV感染者和艾滋病患者 P1基因 16S RRNA基因 

分 类 号：R512.91[医药卫生—内科学]