反硝化除磷菌胞外聚合物的提取及除磷研究  

Extraction of Extracellular Polymeric Substances(EPS) from Denitrifying Polyphosphate-accumulating Organism(DPAO) and Its Action on Phosphorus Removal

作　　者：张倩[1] 李孟[1] 王弘宇[2] 杨开[2] 章彩霞[1] 张超[1] 朱晓云[1] 

机构地区：[1]武汉理工大学土木工程与建筑学院,武汉430070 [2]武汉大学土木建筑工程学院,武汉430072

出　　处：《武汉理工大学学报》2013年第2期130-133,共4页Journal of Wuhan University of Technology

基　　金：国家自然科学基金(51208397;51008239);中国博士后科学基金(2011M501254);武汉理工大学国家级大学生创新创业训练计划(20121049706009)

摘　　要：采用4种方法对反硝化除磷菌ZQN4的胞外聚合物进行了提取试验研究。结果表明,试验中超声波法是一种较好的提取方法。好氧条件下,菌株ZQN4的胞外聚合物中蛋白质含量最高,约占总量的80%。ZQN4对磷的去除率约为89.4%,其中有约88%聚集在菌体内,另外约12%聚集在胞外聚合物中,且大部分以PO34--P的形式存在,表明胞外聚合物对除磷也有一定的贡献。Four extraction methods were adopted to investigate the extraction efficiency of extracellular polymeric sub stances （EPS） from the denitrifying polyphosphate-accumulating organism （DPAO） strain ZQN4 under aerobic condi tion. Experimental results inclicate that protein,amounting to about 80% of total EPS content,was the major component in EPS of strain ZQN4. Ultrasonic method was effective in strain ZQN4 EPS extraction. The removal of phosphorus by strain ZQN4 under aerobic condition was approximately 89. 4%, in which about 880//oo of the reduced phosphorus in the substrate was absorbed by strain ZQN4, while 12% of it was stored in the EPS, indicating the contribution of strain ZQN4 to phosphorus removal. Moreover, the most of reduced phosphorus existed in the form of PO4^3- P.

关 键 词：反硝化除磷菌 胞外聚合物 提取方法 磷吸附 

分 类 号：X506[环境科学与工程—环境工程]