三甲氧苄氨嘧啶胶体金免疫层析检测试纸条的研制  

Study on gold immunochromatography assay for rapid detection of Trimethoprim

作　　者：万宇平 冯才伟 赵正苗 张禹 韩京朋 

机构地区：[1]北京勤邦生物技术有限公司,北京102206

出　　处：《中国乳品工业》2013年第2期24-27,共4页China Dairy Industry

基　　金："十二五"国家科技支撑计划课题(2012BAF14B02)

摘　　要：应用胶体金免疫层析技术建立了一种快速检测食品中三甲氧苄氨嘧啶(TMP)的方法。采用柠檬酸三钠还原法制备胶体金颗粒,标记抗TMP单克隆抗体并冻干于聚苯乙烯微孔中,TMP-OVA和羊抗鼠二抗分别结合于硝酸纤维膜上,依次将样品垫、硝酸纤维膜和吸水纸组装,切割成胶体金试纸条。测试结果表明,TMP快速检测试纸条的灵敏度为50μg/L,检测时间为5 min,特异性高,批内和批间重复性良好,与ELISA试剂盒方法和LC-MS/MS方法符合率为100%。使用简单方便,适合现场快速检测TMP。To establish a rapid assay for detection of Trimethoprim （TMP）, g01d immunochromatography assay （GICA） was studied. Colloidal gold marked coupled with monoclonal antibody against Tfirnethoprim was jet sprayed onto the glass fiber. TMP-OVA and anti-mouse goat immunoglobulins were jet-positioned onto a nitrocellulose membrane. Sample pad, gold jed pad, nitrocellulose membrane and absorbent paper were assembled and cut into detecting card, respectively. The results showed that the sentitive of rapid strip for TMP visual detection limit was 50 μg/L and detection time within 5 rain. Repeatability of strip is good, and the assay gives no false positive and false negative results com- pared with ELISA and LC-MS/MS. So it is very simple and convenient to rapidly detect TMP on the spot.

关 键 词：三甲氧苄氨嘧啶 胶体金免疫层析 试纸条 快速检测 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]