红景天苷对心肺复苏后大鼠心脏功能的保护作用  

Effects of Rhodioside on heart function in rats after cardiopulmonary resuscitation

作　　者：邓节喜[1] 陈劲松[1] 王子敬 郭寿贵[1] 赵蓓[3] 阮发晖[1] 窦燕[1] 林庚海[1] 吴轲[1] 

机构地区：[1]解放军第一七五医院厦门大学附属东南医院心血管内科,福建漳州363000 [2]解放军95883部队卫生教研室,北京102206 [3]解放军总医院心内科超声室,北京100853

出　　处：《中国急救医学》2013年第2期153-156,共4页Chinese Journal of Critical Care Medicine

基　　金：全军医药卫生科研基金（08IMA075）

摘　　要：目的研究红景天苷对复苏后大鼠心脏功能的保护作用。方法60只成年SD大鼠随机分为5组，每组12只：复苏前给药组、复苏即刻给药组、自主循环恢复（ROSC）时给药组、不给药组、假手术组。窒息法建立大鼠心肺复苏模型，按分组在不同时间点给予红景天苷注射液，自主循环恢复维持2h后超声检查大鼠左心室功能。分离培养成年大鼠心肌细胞，给予红景天苷干预后检测钙调神经磷酸酶（CaN—Aβ）、肌浆网钙泵（SERCA2a）的表达。结果与假手术组比较，模型各组左心室功能明显下降（P〈0．05），红景天苷干预组细胞的CaN—Aβ表达下调，SERCA2a表达上调（P〈0．05）。结论红景天苷可以保护复苏后大鼠的心脏功能，可能与调节CaN—Aβ、SERCA2a表达有关。Objective To observe the protective effect of Rhodioside on heart function after cardiopulmonary resuscitation in rats. Methods 60 rats were randomized into 5 groups. Rat models of cardiopulmonary resuscitation were established. Rhodioside was administrated before or after resuscitation at different time. When return of spontaneous circulation sustained for 2 h, heart function was assessed by echocardiography. Cardiac myocytes of adult rats were isolated and cultured with or without Rhodioside, atorvastatin and irbesartan. RT- PCR and Western blot were used to evaluate the expression change of SERCA2a and CaN - AI3. Results Echocardiography revealed that heart function in model groups was significantly lower than that in false surgery group. Further analysis demonstrated that when given Rhodioside before resuscitation, better improvement of heart function could be observed. RT - PCR and Western blot demonstrated that Rhodioside could down - regulate CaN - Aβand up - regulate SERCA2a. Conclusion Rhodioside could improve heart function after resuscitation in rats possibly associated with the regulation of SERCA2a and CaN - Aβ.

关 键 词：红景天苷 心功能 心肺复苏 钙调神经磷酸酶(CaN—Aβ) 肌浆网钙泵(SERCA2a) 

分 类 号：R540.45[医药卫生—心血管疾病]