中药对环境内分泌干扰物的拟雌激素活性拮抗作用  

Traditional Chinese medicine has an effect against the proposed estrogen-like activity of EEDs

作　　者：蔡德培[1] 芦军萍[1] 

机构地区：[1]上海复旦大学附属儿科医院,上海201102

出　　处：《上海医药》2013年第4期33-36,共4页Shanghai Medical & Pharmaceutical Journal

基　　金：国家自然科学基金项目(编号30371820)

摘　　要：目的:采用中药对环境内分泌干扰物(EEDs)暴露水平较高的性早熟患儿进行治疗干预,验证所用中药方案对EEDs拟雌激素活性的拮抗作用。方法:制定针对EEDs拟雌激素活性的中药治疗方案。以该中药制剂治疗73例EEDs暴露水平较高的性早熟女孩,疗程3个月。治疗前后分别测定子宫、卵巢体积、血清雌二醇(E2)、骨矿含量、骨密度及血清骨钙素(OST)含量。并以该中药制剂治疗壬基酚(4-NP)及双酚A(BPA)的染毒动物模型,疗程为14d。治疗前后分别测定其子宫湿重、子宫内膜上皮高度、子宫平滑肌厚度、子宫内膜上皮细胞增殖核抗原(PCNA)的蛋白表达水平及股骨的骨密度。所有指标均进行治疗前后的对比分析,数据处理采用SPSS11.5统计分析软件。结果:治疗后性早熟患儿的子宫体积、血清E2含量及骨矿含量、骨密度、血清OST含量均明显下降(P<0.01);4-NP及BPA染毒动物的子宫湿重、子宫内膜上皮高度、子宫平滑肌厚度、PCNA的蛋白表达水平及股骨骨密度均显著下降(P<0.05)。结论:所制定的中药治疗方案对EEDs的拟雌激素活性具有显著的拮抗作用。Objective： The children with precocious puberty who had exposed to higher pollution of environmental endocrine disruptors （EEDs） were treated with traditional Chinese medicine （TCM）. It was verified that the TCM options had antagonistic effects on proposed estrogen-like activity of EEDs. Method： A TCM treatment options made to proposed estrogen- like activity of EEDs treated 73 girls with Precocious puberty, who had exposed to higher pollution of EEDs and its treatment course was three months. The volumes of uterus and ovarian, and bone mineral density were measured, and serum E2 and osteocalcin（OST） were determined before and after treatment. The animal model contaminated with 4-nonylphenol and bisphenol A were treated with the above mentioned TCM prescription for 14 days. Uterine wet weight, height of the luminal epithelium, thickness of the myometrium and the level of protein expression of proliferating cell nuclear antigen and femoral bone density of the femur in rat uterine were determined before and after treatment. All the indicators were comparatively analyzed, whose data were dealt with SPSS 11.5. Result： After treatment, the volume of uterus, serum E2, BMC, bone density and serum OST all decreased significantly in the girls with precocious puberty （P 〈 0.01）. In the contaminated animal models with 4-nonylphenol and bisphenol A, the uterine wet weight, height of the luminal epithelium, thickness of the myometrium, levels of protein expression of PCNA and femoral bone density descended obviously （P 〈 0.05）. Conclusion： The present therapeutic regime of TCM could have the effect against the proposed estrogen-like activity of EEDs.

关 键 词：环境内分泌干扰物 性早熟 雌激素样活性 中国传统医药 

分 类 号：R285[医药卫生—中药学]