六星黑点豹蠹蛾求偶行为与性信息素产生和释放的时辰节律  

Circadian rhythm of calling behavior and sexual pheromone production and release of the female Zeuzera leuconotum Butler(Lepidoptera: Cossidae)

作　　者：刘金龙[1] 荆小院[1] 杨美红[1] 宗世祥[2] 骆有庆[2] 范丽华[1] 刘红霞[1] 张金桐[1] 

机构地区：[1]山西农业大学化学生态研究所,太谷030801 [2]北京林业大学省部共建森林培育与保护教育部重点实验室,北京100083

出　　处：《生态学报》2013年第4期1126-1133,共8页Acta Ecologica Sinica

基　　金：山西省自然科学基金项目(2010011042-2);国家"十二五"科技支撑计划项目(2012BAD19B07)

摘　　要：六星黑点豹蠹蛾Zeuzera leuconotum Butler(Lepidoptera:Cossidae)是一种重要的园林害虫。为研究利用其性信息素防控六星黑点豹蠹蛾,在光周期L∶D=14∶10、温度19—32℃、相对湿度75%—85%条件下,对雌蛾求偶行为进行了观察;采用腺体提取法和瓶内收集法分别制备了不同日龄在同一时辰、同日龄在不同时辰的雌蛾性信息素粗提物,然后测定雄蛾触角对各种性信息素提取物的触角电位反应;以雄蛾对腺体提取物的EAG值表示性信息素的产生量,对瓶内收集物的EAG值表示释放量,检测性信息素产生与释放的时辰节律。结果表明:雌蛾求偶均发生在暗期;雌蛾求偶率与日龄有关,其中2日龄雌蛾求偶率最高,平均为86.7%;不同日龄雌蛾求偶高峰期均在进入暗期4—6h期间;随着虫龄的增加,求偶高峰期前移。雌蛾羽化当晚体内即可产生性信息素,并且向体外释放,但量较少;2日龄雌蛾产生和释放的性信息素量最大;雌蛾腺体中性信息素含量从进入暗期后逐渐增加,在暗期5h含量最高,随后逐渐减少;雌蛾释放性信息素从暗期4h开始,至暗期6h释放量最大。通过林间处女雌蛾诱捕试验证明了六星黑点豹蠹蛾的求偶行为与性信息素产生和释放在时辰节律上具有一致性。Zeuzera leuconotum Butler（Lepidoptera： Cossidae） is one of the most damaging pests on forestry in northern China in recent years.Because its larvae cause damage by tunneling into tree trunks,it is very difficult to control by cutting off the damaged branches or applying insecticides.In order to find a pollution-free and efficient controlling method,we have already begun a series of studies on the sex pheromone of the pest.It is necessary to know the character of calling behavior and the circadian rhythm of sex pheromone production and release by female Z.leuconotum. In this paper,the female moth calling behavior and characters were investigated through observation in the insectariums under the condition of（19—32）℃,75%—85% RH and 14L∶10D photoperiod.And the circadian rhythm of sex pheromone production and release by the female moth was researched in detail by determining the electrophysiological（EAG） responses.The crude extracts of sexual pheromone were prepared by the two methods.One of the methods was to directly extract pheromone from female sexual gland by hexane,and another was to collect pheromone from air in the bottle which filled with a female moth.The Former reflected the sex pheromone production,and the latter reflected the sex pheromone release. The results showed that the calling behavior of all female moths occurred during the scotophase.Some females（43.3%） called on the first night after eclosion and called mainly during the second half of the scotophase.Calling varied with age of female.The percentage of females calling increased from 1 to 2-d-old and decreased from 3 to 6-d-old gradually.The percentage of 2-d-old females calling was the biggest（86.7%,average）,significantly bigger than 1 or 4,5,6-d-old females（P0.05）.The old females（5 to 6-d-old） called earlier than younger in the scotophase.The calling peak occurred in 4-6 h of scotophase. The EAG response of males to different extracts reflected that only a few females could produce and release sex pheromone

关 键 词：六星黑点豹蠹蛾 求偶行为 性信息素 产生与释放 时辰节律 

分 类 号：S433[农业科学—农业昆虫与害虫防治] Q968[农业科学—植物保护]