设施番茄长期连作土壤微生物群落结构及多样性分析  

Effect of Long-term Continuous Cropping of Protected Tomato on Soil Microbial Community Structure and Diversity

作　　者：马宁宁[1] 李天来[1] 

机构地区：[1]沈阳农业大学园艺学院,辽宁省设施园艺重点实验室,沈阳110866

出　　处：《园艺学报》2013年第2期255-264,共10页Acta Horticulturae Sinica

基　　金：‘十二五’国家科技支撑计划项目(2011BAD12B03);现代农业产业技术体系建设专项资金项目(CARS-25);辽宁省重大科技攻关项目(2011215003);沈阳市农业科技发展专项(F11-092-3-00)

摘　　要：利用PCR-DGGE技术,研究了裸地及不同连作年限的设施番茄栽培土壤中细菌和真菌的群落结构及多样性的变化情况。结果表明:设施条件下种植番茄明显改变了土壤土著细菌的群落结构,但连作年限对土壤细菌多样性影响较小,细菌群落结构变化不大;土著真菌的群落结构稳定性优于土著细菌,但土壤真菌的优势种群在不同连作年限的土样中变化较大,连作显著降低了某些真菌的数量,同时显著增加了另一些真菌的数量,其中,连作20年番茄的土壤真菌新出现的优势种群最多,且与非优势种群的真菌数量差异较大,这种番茄连作后改变土壤真菌种群平衡的现象可能是导致番茄产生连作障碍的重要原因之一。通过电泳条带的回收、测序,可知所取土样中的细菌优势种群属于Clostridium butyricum、Uncultured bacterium、Bacillus和Arthrobacter,真菌优势种群属于Saccobolus dilutellus、Uncultured fungus、Lasiobolusciliatus、Spooneromyces laeticolor和Lasiobolidium orbiculoides。Community structure and diversity of microorganism in bare soil and continuous cropping protected tomato field were studied with PCR-DGGE technology. Results showed that soil indigenous bacterial community structure was significantly influenced by planting tomato under protected field, but soil bacterial community structure and diversity were not sensitive to continuous cropping years. Compared with soil indigenous bacteria, indigenous fungal community structure was more steady, but the dominant fungi were more different in the fields with different continuous cropping years, continuous cropping significantly decreased the number of some fungi, meanwhile, the number of some other fungi was significantly increased, most dominant fungi populations were enriched in soil with tomato continuous cropping years of 20, and which number was quite different with the non-dominant fungi populations. The change of soil fungi population balance that caused by tomato continuous cropping maybe one of the important reasons for the tomato continuous cropping obstacle. Recycling and sequence analysis Of prominent electrophoretic bands showed that the dominant bacteria of soil sample belong to Clostridium butyricum, Uncultured bacterium, Bacillus and Arthrobacter, while the dominant fungus belong to Saccobolus dilutellus, Uncultured fungus, Lasiobolus ciliatus, Spooneromyces laeticolor and Lasiobolidium orbiculoides.

关 键 词：番茄 长期连作 土壤微生物 PCR-DGGE 群落结构 多样性 

分 类 号：S641.2[农业科学—蔬菜学]