利用废弃胚胎进行体外受精实验室质控的可行性探讨  

Feasibility of discarded embryos as a quality control in the IVF laboratory

作　　者：沈肖方[1] 张玉花[1] 陈丽丽[1] 郝翠芳[1] 

机构地区：[1]山东青岛大学医学院附属烟台毓璜顶医院生殖中心实验室,烟台264000

出　　处：《生殖医学杂志》2013年第2期97-100,共4页Journal of Reproductive Medicine

摘　　要：目的探讨废弃胚胎作为体外受精(IVF)实验室质控的可行性。方法通过囊胚序贯培养将无原核(0PN)、单原核(1PN)、3原核(3PN)和发育迟缓或停滞的2原核(2PN)的废弃胚胎进行囊胚培养,比较不同来源胚胎的囊胚形成情况。结果共收集396个废弃胚胎,经序贯培养形成98个囊胚(24.75%),0PN、2PN、1PN、3PN的囊胚形成率分别为50.50%(51/101)、24.59%(30/122)、1 7.07%(7/41)、7.58%(10/132),其中0PN、停滞的2PN的胚胎有孵出,孵出率分别为47.06%(24/51)和33.33%(10/30);有囊胚形成与无囊胚形成患者的临床妊娠率分别为30.28%(43/142)、1 9.72%(28/142),两者相比有统计学差异(P<0.05);特别是0PN+2PN患者的临床妊娠率为23.24%(33/142),显著高于无囊胚形成者的9.15%(13/142)(P<0.01)。结论废弃胚胎有不同程度的发育潜能,可部分发育成囊胚,特别是0PN+2PN囊胚形成率超过35%,可以作为IVF实验室质控的一种辅助方法,并可作为临床妊娠结局预测的参考指标。Objective： To study the feasibility of discarded embryos as a quality control in the IVF laboratory. Methods： The embryos from 0PN, IPN, and 2PN were cultured in microdrops of sequenced medium and observed daily from Day 3 to Day 6. Results. A total of 396 abandoned embryos were cultured. Ninety eight blastocysts were formed from Day 4 to Day 6 and the blastocyst rate was 24.75%. The blastocyst formation rate from 0PN, 1PN, 2PN, 3PN was 50.5%（51/101）, 24.59%（30/122）, 17.07%（7/41）and 7.58%（10/132）, respectively. The zy- gote with 2PN and 0PN could develope to embryos. The hatch rate was 30%（10/30）and 47.06%（24/51）, respectively. The clinical pregnancy rate of the women with blastosphere formed was 30.98% （43/142）, which was significantly higher than those without blastosphere formed（19.71%, 28/142）（P〈0.05）. The clinical pregnancy rate of the women with 0PN and 2PN was 23.24% （33/142）, which was significantly higher than those without formation of blastosphere（9.15%, 13/142）（P%0.01）. Conclusions： The discarded embryos have different development potential. They can develop into blas- tospheres. The blastosphere formation rate of 0PN and 2PN can be more than 35 %. It can be used as a quality control for IVF laboratory and a predictor for clinical pregnancy outcome.

关 键 词：废弃胚胎 囊胚 异常受精 质量控制 

分 类 号：R4[医药卫生—临床医学]