广东地区人微小病毒B19母婴传播及异常胎儿和新生儿人微小病毒B19感染的研究  

THE STUDY OF HUMAN PARVOVIRUS B19 INFECTION IN PERINATAL TRANSMISSION AND ABNORMAL FETUSES AND NEONATES IN GUANGDONG

作　　者：曹虹[1] 钟梅[2] 张文炳[1] 王香云[1] 

机构地区：[1]第一军医大学微生物学教研室,广州510515 [2]第一军医大学南方医院妇产科

出　　处：《中国人兽共患病杂志》2000年第6期3-5,共3页Chinese Journal of Zoonoses

基　　金：广东省自然科学基金

摘　　要：目的　为研究人微小病毒 B19在广东地区母婴及异常胎儿和新生儿中的感染状况。方法　应用 PCR方法检测70 0份孕妇外周血和新生儿脐血 ,PCR结合限制性内切酶分析以及原位杂交检测 2 4份异常胎儿和新生儿组织。结果　孕妇和胎儿血清中 B19病毒的检出率分别为 1.14% (4/ 35 0 )和 0 .2 8% (1/ 35 0 ) ;17例异常胎儿和 7例新生儿中 ,分别有 5份胎儿和 3份新生儿组织 PCR结果阳性。PCR产物经限制性内切酶 Hae 酶切分析 ,证实为 PVB19特异的 DNA片段。用原位杂交的方法 ,在部分异常胎儿的脑或脾组织小血管内或周围的原始红细胞核中检测到 DNA阳性颗粒。结论　广东地区孕妇 B19病毒的感染可引起母婴传播 ,并可能对胎儿造成一定的危害 ,但其感染率很低。在评估 B19病毒感染时 ,应有可靠方法区分现症感染和既往感染。This study was undertaken to investigate mother-to-infant transmission of human parvovirus B19 and the significance of prevalence of B19 virus in abnormal fetuses in Guandong.The polymerase chain reaction (PCR)was established to detect parvovirus B19 DNA in 700 sera from 350 maternal-infant pair groups.The prevalence of B19 virus DNA was 1.14%(4/350)and 0.28%(1/350)in the sera of pregnant women and cord blood of their neonates respectively.Parvovirus B19 DNA sequences were also detected in abnormal fetuses and new-born by PCR.The positive results were obtained in 5 samples of fetal tissues from 17 abnormal fetuses and in 3 those of neonatal tissues from 7 cases of neonatal death.The amplified products of PCR were identified to be the target DNA with HaeⅢdigestion.By in situ hybridization,parvovirus DNA could be detected mainly in the nuclei of immature hematopoetic cells within fetal brain or spleen whose PCR tests were positive.The study results suggest that human parvovirus B19 infection does exist in maternal-infant transmission in Guangdong and might lead to harm on fetuses,but the prevalence rate of B19 virus may be very low.The evaluation of B19 virus infection might depend on reliable assay to determine present infection or past infection. \ \

关 键 词：人微小病毒B19 母婴传播 原位杂交 聚合酶链反应 

分 类 号：R714.251[医药卫生—妇产科学] R373.9[医药卫生—临床医学]