问号赖型钩端螺旋体23kDa蛋白的免疫原性研究  

STUDY ON THE IMMUNOGENICITY OF 23KDA ANTIGEN OF LEPTOSPIRA INTERROGANS SEROVAR LAI

作　　者：刘洪斌[1] 戴保民[2] 章涛[3] 李胜富[2] 方之茂[2] 

机构地区：[1]宁波大学医学院,宁波315211 [2]华西医科大学钩研室 [3]福建医科大学分子医学教研室

出　　处：《中国人兽共患病杂志》2000年第6期54-55,12,共3页Chinese Journal of Zoonoses

摘　　要：目的　探讨 p DL12 1的 1.0 kb0 17株问号赖型钩端螺旋体 DNA片段可否作为钩体重组疫苗广谱保护性抗原的候选。方法　采用 SDS- PAGE制备 2 3k Da蛋白 ,免疫日本大耳兔制备抗 2 3k Da抗血清 ,Western blot鉴定其免疫原性。结果　2 3k Da重组抗原兔抗血清可识别 p DL12 1体外表达 2 3k Da抗原产物和问号赖型钩端螺旋体 0 17株超声抗原成份 ;用 Westemblot鉴定抗血清效价 ,其抗体滴度为 1/ 12 80 0 ;用 p DL12 1重组质粒主动免疫豚鼠 ,可使豚鼠抵抗强毒力株钩端螺旋体攻击 ,表现出免疫保护作用。结论　该重组 2 3k Da抗原有良好的免疫原性 ,可能是赖型钩体 0 17株的保护性抗原。In order to test whether the 23kDa protein could be the candidate for new generation vaccines against leptospirosis for the developing world,the isolated 23kDa protein band was cut and injected twice into rabbits to raise anti-23kDa serum. What is more,the protection of E.coli lysate of pDL121 on guinea pigs was done.The results showed that the anti-23kDa serum could recognize the sonicated antigen of L.interrogans serovar lai strain 017 and 23kDa protein expressed in pDL121 and the tilter of the antiserum were very high,approximately 1/12800.Injection of the E.coli lysate of pDL121 with Freund's adjuvant into guinea pigs resulted in some protection of the animal against the challenge with strain 017.This indicated that 23kDa had good imunogenicity and could serve as a candidate for protective antigen of L.interrogans.

关 键 词：问号赖型钩端螺旋体 免疫印迹 抗原 DNA 

分 类 号：R514.4[医药卫生—内科学] R377.5[医药卫生—临床医学]