阿苯哒唑及其代谢物对猪蛔虫肌肉线粒体酶复合体活性作用的研究  

PRELIMINARY STUDIES OF ALBENDAZOLE(ABZ),ALBENDAZOLE-SULFOXIDE (ABZSX) AND AIBENDAZOLE-SULFONE(ABZSN) ON THE ENZYME COMPLEXES ACTIVITIES IN MITOCHONDRIA OF ASCARIS SUUM MUSCLE

作　　者：赵冠宏[1] 许炽标[1] 辛文芬[2] 

机构地区：[1]北京热带医学研究所,北京100050 [2]中国预防医学科学院劳动卫生与职业病研究所

出　　处：《中国人兽共患病杂志》2000年第6期74-75,共2页Chinese Journal of Zoonoses

摘　　要：目的　初步了解阿苯哒唑 (albendazole,ABZ)及其主要代谢物亚砜 (albendazole- sulfoxide,ABZSX)和砜(albendazole- sulfone,ABZSN)对猪蛔虫的作用机制。方法　应用紫外分光光度计扫描系统对猪蛔虫肌肉线粒体中四种酶复合体 (com plex - NADH细胞色素 C脱氢酶 ;complex —琥珀酸 Co Q脱氢酶 ;complex — Co Q细胞色素还原酶 ;complexIV—细胞色素 C氧化酶 )活性进行测定 ,以鼠肝线粒体作对照 ,再经 ABZ及其代谢物 ABZSX,ABZSN作用后观察其活性改变。结果　 ABZ及其代谢物 ABZSX对猪蛔虫肌肉线粒体中琥珀酸脱氢酶 (complex )的活性有明显的抑制作用 ,而对鼠肝线粒体中四种酶复合体均无明显抑制作用。结论　 ABZ及其代谢物主要抑制了蛔虫肌肉线粒体中琥珀酸脱氢酶的活性 ,使其不能完成呼吸链的氧化磷酸化过程 ,从而达到杀虫效果。Aim\ To study the mechanism of ABZ,ABZSX and ABZSN on Ascaris Suum.Methods\ The activities of enzyme complexes in mitochondria were detected by spectrophotometer for the study of effects of ABZ,ABZSX and ABZSN on the anaerobic respiratory chain of enzyme complexes in mitochondria of Ascaris Suum muscle and rat liver.Results\ The activity of succinate CoQ reductase in Ascaris muscle mitochondria was apparently suppressed by ABZ,ABZSX.Conclusion\ Preliminary study on the mechanism and toxicity of ABZ through enzyme studies,in order to find a more effective and satisfactory drug with low toxicity for clinical use.

关 键 词：线粒体 酶复合体活性 蛔虫肌肉 阿苯哒唑 治疗 

分 类 号：R532.110.5[医药卫生—内科学]