OsELF3 Is Involved in Circadian Clock Regulation for Promoting Flowering under Long-Day Conditions in Rice  

作　　者：Ying Yang Qiang Peng Guo-Xing Chen Xiang-Hua Li Chang-Yin Wu 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan,430070, China

出　　处：《Molecular Plant》2013年第1期202-215,共14页分子植物（英文版）

基　　金：This work was supported by National Natural Science Foundation of China Grant 30970172, 863 Project Grant 2012AA10A303, and the Program for New Century Excellent Talents in University.

摘　　要：Heading date is a critical trait that determines cropping seasons and regional adaptability in rice （Oryza sativa）. Research efforts during the last decade have identified some important photoperiod pathway genes that are conserved between Arabidopsis and rice. In this study, we identified a novel gene, Oryza sativa ELF3 （OsELF3）, which is a putative homolog of the ELF3 gene in Arabidopsis thaliana. OsELF3 was required for the control of heading date under long-day conditions. Its Tos17-tagging mutants exhibited a delayed heading date phenotype only under long-day, but not short-day, conditions. OsELF3 was highly expressed in leaf blades, and the OsELF3 protein was localized in the nucleolus. An obvious diurnal rhythm of OsELF3 transcript level was observed, with a trough in the early day and a peak in the late night in wild-type plants. However, this expression pattern was disrupted in oself3 mutants. Further inves- tigations showed that the expression of OsGI and Ghd7 was up-regulated in the oself3 mutant, indicating that OsELF3 acts as a negative regulator upstream of OsGI and Ghd7 in the flowering-time control under long-day conditions. The rhythmic expression of circadian clock-related genes, including some OsPRR members, was obviously affected in oself3 mutants. Our results indicated that OsELF3 acts as a floral activator in the long-day photoperiodic pathway via its cross- talk with the circadian clock in rice.Heading date is a critical trait that determines cropping seasons and regional adaptability in rice （Oryza sativa）. Research efforts during the last decade have identified some important photoperiod pathway genes that are conserved between Arabidopsis and rice. In this study, we identified a novel gene, Oryza sativa ELF3 （OsELF3）, which is a putative homolog of the ELF3 gene in Arabidopsis thaliana. OsELF3 was required for the control of heading date under long-day conditions. Its Tos17-tagging mutants exhibited a delayed heading date phenotype only under long-day, but not short-day, conditions. OsELF3 was highly expressed in leaf blades, and the OsELF3 protein was localized in the nucleolus. An obvious diurnal rhythm of OsELF3 transcript level was observed, with a trough in the early day and a peak in the late night in wild-type plants. However, this expression pattern was disrupted in oself3 mutants. Further inves- tigations showed that the expression of OsGI and Ghd7 was up-regulated in the oself3 mutant, indicating that OsELF3 acts as a negative regulator upstream of OsGI and Ghd7 in the flowering-time control under long-day conditions. The rhythmic expression of circadian clock-related genes, including some OsPRR members, was obviously affected in oself3 mutants. Our results indicated that OsELF3 acts as a floral activator in the long-day photoperiodic pathway via its cross- talk with the circadian clock in rice.

关 键 词：heading date photoperiod pathway circadian clock rice. 

分 类 号：S626[农业科学—园艺学] Q811.213[生物学—生物工程]