金刚健骨片调节整合素β_1和α_Ⅴβ_3表达水平的实验研究  

Effect of Jingang Jiangu pill(金刚健片) on expression of integrin β_1 and α_Ⅴβ_3 in ovariectomized osteoporosis model rats

作　　者：杨少锋[1] 李玲慧[2] 陈青[1] 姚共和[1] 邓博[1] 向剑锋[1] 聂颖[1] 罗振华[1] 郭彦涛[1] 

机构地区：[1]湖南中医药大学附属第一医院,湖南长沙410007 [2]上海中医药大学附属曙光医院石氏伤科医学中心,上海201203

出　　处：《中国骨伤》2013年第2期138-141,共4页China Journal of Orthopaedics and Traumatology

基　　金：国家教育部新教师基金项目资助(编号:20094323120005)~~

摘　　要：目的:通过对去势大鼠整合素表达水平的观察,揭示金刚健骨片治疗骨质疏松症的作用机制。方法:将50只10月龄Wistar雌性大鼠随机分为5组:福善美组(FSM)、金刚健骨片组(JGJG)、骨松宝颗粒组(GSB)、模型组(OVX)、假手术组(SHAM)。治疗组及模型组行卵巢摘除术,假手术组切除相同重量的肠系膜。统一饲养13周造模成功后,治疗组分别以相应药物连续给药13周,JGJG组每只大鼠每天给药0.13g,GSB组每只每天给药0.86g,FSM组每只每天给药0.28mg,模型组及假手术组以生理盐水替代。治疗结束后处死动物取出骨组织,进行骨密度测定,并利用免疫组化法测定整合素αⅤβ3及β1表达水平。结果:骨密度测定结果显示,治疗组的股骨密度、整合素β1均较OVX组有明显提高;治疗组整合素αⅤβ3明显低于OVX组。结论:金刚健骨片能显著提高去势大鼠的股骨密度、整合素β1的表达水平,并可降低整合素αβ的阳性表达率,从而干预成骨-破骨细胞偶联,达到治疗目的。Objective：To investigate the regulatory effect of Jingang Jiangu pill （金刚健片, JGJG） on expression of inte- grin in ovariectomized rats. Methods：Fifty ovariectomized 10 months old female rats were randomly divided into 5 groups： Fushanmei group （FSM） ,Jingang Jiangu pill （ 金刚健片）group （JGJG） ,Gusongbao granule group （GSB） ,Model group （OVX）, Sham group. After ovariectomized,the rats were raised in the same environment for 13 weeks. The rats in JGJG group took 0.13 g JGJG pill orally each day for each rat;the rats in GSB group took 0.86 g GSB granule orally each day for each rat; the rats in FSM group took 0.28 mg FSM orally each day for each rat;and the rats in OVX and sham groups took sodium. The treatment duration of rats in above 5 groups was 13 weeks. Bone mineral density （BMD） and the expression of integrin β1 and αvβ3 were detected in each group after the treatment. Results：The BMD and the expression of integrin β1 in FSM group, JGJG group and GSB group improved obviously than that of OVX group. There were statistical difference between these groups （P〈0.05）. The expression of integrin αvβ3 of the three treating groups significantly depressed. Conclusion ：The JGJG pill im- proves BMD and express of integrin β1 in ovariectomized rats and reduces express of integrin αvβ3 through the regulation of the coupling of osteoblasts and osteoclasts.

关 键 词：骨质疏松 整合素类 卵巢切除术 骨密度 

分 类 号：R392.1[医药卫生—免疫学]